IBA GmbH
Immobilisation with Near Covalent Binding Affinity
Twin-Strep-tag™ Capture Kit for SPR Experiments
- Indirect immobilisation of the ligand prevents the ligand to be inactivated or influenced by the immobilisation procedure
- High affinity in low pM range enables long term measurements (T1/2 = 13 h)
- Chip can be easily regenerated by an established procedure
- Ligands can be captured directly from complex samples due to the high affinity and specificity of the Twin-Strep-tag:Strep-Tactin XT interaction
Try our New High Affinity Strep-tag™ Technology
Strep-Tactin XT 4Flow™ Starter Kit consists of:
- Strep-Tactin XT 4Flow gravity flow column
- Buffer W (Strep-Tactin/Strep-Tactin XT Wash Buffer)
- Buffer BXT (Strep-Tactin XT Elution Buffer with Biotin)
- Buffer XT-R (Strep-Tactin XT Regeneration Buffer with Biotin MgCl2)
- Strep-Tactin conjugated with HRP (for Western blot detection)
- Short protocol
Exosome Isolation - Quick and Simple
The Fab-TACS™ Exosome Isolation Technology offers:
- Highly specific and reproducible isolations with minimal contamination in a source-independent manner
- Quick and simple protocol, without magnetic beads or ultracentrifugation, no extra device needed
- Label-free exosomes with intact biological functions due to reversible reagents
Traceless Affinity Cell Selection
Fab-TACS™ technology perfectly combines the benefits of positive and negative cell selection
- High purity, yield and viability
- Label-free, non-activated cells
- From whole blood, buffy coat, PBMCs or murine samples
- Manual or automated cell separation
Magnetic Cell Selection
- Fully reversible
- No stimulation of cells after cell separation
- Optimal preservation of cell effector function
- Improved viability of cells
Cell Stimulation & Expansion
- Completely reversible reagents
- Termination of stimulation at any given point in time
- Adjustable CD3 : CD28 ratio
- Bead-free
Peptides for T cell stimulation
- Purity > 85 %
- Endotoxin free
Fluorescent Cell Staining
Detection of antigen-specific T cells with MHC I Streptamers™ and of receptor-specific T cells with Fab Streptamers™
- Fully reversible
- High specificity, sensitivity and reproducibility
- No leftover of staining reagents on the cells after FACS sorting
- Optimal preservation of cell effector function
- Improved viability of cells
Cloning
More than 150 Twin/Strep-tag™ II expression vectors
- Variety of Twin/Strep-tag II combined with His, FLAG or GST
- Different N-/C-terminal orientations
- Expression hosts: E. coli, mammalia, insect cells, yeast
- Easy cloning via StarGate vectors
Mammalian Expression
The MEXi mammalian expression system
Optimised for efficient protein expression and Strep-tag purification in mammalian cells.
- Perfectly matched HEK293E cell line and cell culture media
- Transient expression for time and cost efficient protein production
- Multiple expression vectors from IBA with different features
Protein Purification
Strep-tag™ technology from the original manufacturer
- High affinity in low pM range
- Rapid one-step purification with unparalleled protein purity (> 95 %)
- Mild and specific elution
- Tolerates broad range of buffer conditions
- Suitable for metalloproteins, membrane proteins, low abundant proteins, protein complexes and any other
Detection
For Western blot analysis, immunofluorescence, FACS and ELISA
Two different detection systems are provided:
- Strep-Tactin and Strep-Tactin XT conjugate for Twin/Strep-tag II proteins
- Monoclonal antibodies against Twin/Strep-tag II
Immobilisation
Near covalent binding affinity – Immobilisation by Strep-Tactin XT: Twin-Strep-tag for screenings, diagnostic assays, protein biochemistry, protein kinetics and interaction studies, SPR experiments (Biacore) or BLI
- High affinity in low pM range enables measurements over a long time
- Minimal non-specific binding
- Minimal coefficients of variation (cv)
- Chip for SPR experiments can be easily regenerated
Traceless Affinity Exosome Isolation
Quick and simple isolation of unlabelled exosomes using Fab-TACS™ technology
The Fab-TACS technology for exosome isolation offers
- Highly specific and reproducible isolations
- Minimal contamination by other EVs
- Label-free exosomes with intact biological functions
- Source-independent application
- Reversible capture and release of exosomes
- A quick and simple protocol, without magnetic beads or ultracentrifugation
Cell Selection & Expansion
- Application Note FABian Lymphocyte Isolation
- Application Note Central memory T-cells isolated and stained with CD8α and CD62L Fab Streptamer reagents
- Application Note Combining MHC-I and CD8+ Fab Streptamer reagents
- Application Note Regulatory T-cells isolated and stained with CD4-CD25 and CD45RA Fab Streptamer reagents
- Application Note CD3-T Lymphocytes isolated and stained with Streptamer reagents
Exosomes
About IBA GmbH
Solutions For Life Sciences
IBA Lifesciences provides innovative technologies for life science applications. The product portfolio includes tools for cell selection and expansion as well as protein production and assays.