IBA GmbH™ CD3 Fab Streptamer™ Isolation Kit MB

Description

• Strep-Tactin™ Magnetic Microbeads for Fab Streptamers™; 2 vials for 5 x 10⁸ cells each
• CD3 Fab-Strep, human for isolation of T-cells; 1 vial for 1 x 10⁹ cells
• Biotin stock solution for Streptamer™ technology; 2 x 1mL
• Buffer IS, Streptamer™ Washing Buffer, 10x concentrated; 50mL
• Microbeads are intended for use with a permanent magnet for manual cell isolation; we recommend our StrepMan magnet for 2 x 15mL and 2 x 50mL Falcon tubes.
• Principle of Selection: Isolation of human CD3+ T cells is performed by positive selection. PBMCs are labeled with magnetic CD3 Fab Streptamers™. Labeled cells are isolated in a strong magnet (StrepMan, horizontal position) where they migrate toward the tube wall on the side of the magnet. This CD3 positive cell fraction is collected and cells are liberated from all labeling reagents by addition of biotin in a strong magnet (StrepMan, upright position). The magnetic Streptamers™ migrate toward the tube wall and the label-free cells remain in the supernatant. Biotin is removed by washing.
• The resulting cell preparation is highly enriched with CD3+ T cells with a purity of more than 90%. No depletion steps and no columns are needed.