IBA GmbH™ CD45RA Fab Streptamer™ Isolation Kit MB

Description

• Strep-Tactin™ Magnetic Microbeads for Fab Streptamers™; 2 vials for 5 x 10⁸ cells each
• CD45RA Fab-Strep, human for isolation of T-cells; 2 vials for 5 x 10⁸ cells
• Biotin stock solution for Streptamer™ technology; 2 x 1mL
• Buffer IS, Streptamer™ Washing Buffer, 10x concentrated; 50mL
• Microbeads are intended for use with a permanent magnet for manual cell isolation; we recommend our StrepMan magnet for 2 x 15mL and 2 x 50mL Falcon tubes.
• Principle of Selection: Isolation of human CD45RA T cells is performed by positive selection. PBMCs are labeled with magnetic CD45RA Fab Streptamers™. Labeled cells are isolated in a strong magnet (StrepMan, horizontal position) where they migrate toward the tube wall on the side of the magnet. This CD45RA positive cell fraction is collected and cells are liberated from all labeling reagents by addition of biotin in a strong magnet (StrepMan, upright position). The magnetic Streptamers™ migrate toward the tube wall and the label-free cells remain in the supernatant. Biotin is removed by washing.
• The resulting cell preparation is highly enriched with CD45RA+ T cells with a purity of more than 90%. No depletion steps and no columns are needed.