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Gibco™ ExpiSf™ Expression System Starter Kit
Gibco™ ExpiSf™ Expression System Starter Kit is a baculovirus-insect cell protein expression system for superior yields and consistent performance run after run using a fast, streamlined workflow.
Brand: Gibco™ A38841
Additional Details : Weight : 0.05000kg
Description
- A chemically defined, yeastolate-free, animal origin-free medium for consistent cell growth and protein expression
- Integrated components that work in concert to deliver three times higher protein yields compared to traditional insect expression platforms
- Robust production of high-titer, high-quality P0 recombinant baculovirus in suspension culture format without the need for further virus amplification
- A fast, simplified workflow that allows you to obtain protein in as little as 6-10 days; half the time of traditional protocols
- Scalable expression that can be applied to cultures from 24-deep well plates to >3L shake flasks
Specifications
| Sf9, SFM Adapted | |
| Baculovirus | |
| Polyhedrin | |
| Approved for shipment at Room Temperature or on Wet or Dry Ice |
| • 1000 mL bottle ExpiSf CD Medium, store in the dark at 2–8°C • 4 x 1 mL ExpiSf Enhancer, 2–8°C • 1 mL ExpiFectamine Sf Transfection Reagent, 2–8°C • 2 x 1 vial ExpiSf9 Cells, store in vapor-phase liquid nitrogen • 500 mL Opti-MEM, 2–8°C • 10 µg pFastBac1 vector, 2–8°C • 4 ng pFastBac1-Gus control vector, 2–8°C • 5 x 0.1 mL MAX Efficiency DH10Bac Competent Cells, –80°C • 24 x 125-mL Single-Use Nalgene PETG Erlenmeyer Flasks, store at room temperature |
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| ExpiSf™ | |
| Untagged | |
| Restriction Enzyme ⁄ MCS |