Thermo Scientific™ Power-Bind™ Streptavidin Coated Particles

Description

The use of microparticles as a solid phase support in various immunoassays and affinity purifications has been known for some time; however, achieving high activity and stable binding of solid phase ligands has been a major difficulty. Biotinylated compounds become stably bound to Power-Bind Streptavidin Coated particles after simple incubation in buffer due to the high affinity of the biotin-streptavidin interaction. The particles also function as a spacer, which improves the specific activity of the bound ligand. Monodisperse nonmagnetic particle suspensions with streptavidin covalently bound to the surface in a highly active form

Features:

• Made from 0.3μm or 0.8μm Thermo Scientific™ OptiLink™ Carboxylate-Modified particles
• High biotin-binding capacity
• Extremely stable, long shelf life
• Simplifies the process of binding ligands to particles
• Combines the advantages of high surface area and easy, high affinity, high specific activity binding
• Excellent for chemiluminescent assays, biotinylated ligand capture, and for molecular biology, immunoassay, or sample prep applications

Benefits:

• Dissociation constant (Kd 10-15 molar), easy one-step binding protocols for biotinylated ligands
• High activity of surface bound ligands to easily solve difficult coupling problems
• Simple aqueous biotinylation reactions
• Low nonspecific interactions (blocked microparticle surface)
• Highly mobile particles for membrane-based applications
• Can be used in EIA formats with biotin/enzyme detection systems
• Beneficial spacer effect of SA molecule
• A choice of 0.3μm or 0.8μm diameters for different application requirements

Recommended for:

• Universal nonmagnetic bead for coupling unique ligands.
• Clinical diagnostics and molecular biology applications.