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Techne Prime Pro 48 Real-time qPCR System
A new, high specification real time thermal cycler that features highest thermal block uniformity and fastest run time of any block-based qPCR system on the market. Accommodates a unique 48-well polypropylene PCR plate utilizing the same geometry as standard 384-well plates, but only 1/8 of the size.
Brand: Techne PRIMEPRO48
Additional Details : Weight : 15.00000kg
Includes: Dock and Squeegee, EcoControl and EcoStudy software USB stick, Pack of 50 Plates, Pack of 50 Plate seals, Power and Ethernet Cables
Description
Speed
- Ramp rate average 5.5°C/sec. Fast heating and cooling
- 40 cycles in 40 minutes before optimisation
- 40 cycles in 15 minutes when optimised
- Genotype in 4 minutes with over 99% accuracy
- Process up to 40% more samples per hour than a traditional 96-well instrument
- Set up a 4plex reaction in only four mouse clicks
- MIQE guidelines compliant software
Thermal system
- High resolution melt (HRM) capability
- Proprietary thermal system. Hollow fluid-filled silver block anodised with gold
- Up to 5x uniformity
Reagents
- Instant settle time to achieve world leading uniformity of ±0.1°C across the block - any well, any instrument - consistent results in any system
- 400 analyte specific qPCR reagent kits available
- Accepts all popular brands of reagents
Advanced Optical Technology
- Four colours for easy multiplexing
- Proprietary optical system utilised Adaptive LED Control
- Automatically normalizes fluorescence. Never saturate the detector. Eliminate cross talk
- Always reads all 48 wells, with all 4 filters at every cycle
ProDock
- Ergonomic plate loading dock allow for easy loading of the plate and is backlit to allow the user to easily visualise sample loading
- Base with LED backlight
- Plate adapter fits standard centrifuge rotors
- Includes plate sealing tool
Performance
- Consistent results provide trust and confidence. Well-to-well, run-to-run , machine-to-machine
- Compile data from multiple experiments. Pool plates and results across labs and instruments
- Record every well, with every filter, in every cycle. Never miss a data point
- Adaptive LED Control means you never have uncertainty of saturated unknowns, no bleaching though light to neighbouring wells or maximising the wide dynamic range
Sensitivity
- ±0.1°C uniformity across the whole block instantly after every temperature change means that any well, in any instrument, provides same answer
- HRM - Identify Class IV SNP >99.9% of the time
- Unsurpassed block uniformity with proprietary block technology enables the researcher to use duplicates on Prime Pro 48
Pro Software
- Easy to use software that integrates user control, real-time data collection, and advanced data analysis
- New software version 5 enables a widened chemistry library and contains new thermal profiles
- Supplied on a USB stick
- Uses a unique icon-driven user interface to simplify experimental design and setup
ProStudy software
- Data analysis and Multi-Experiment Analysis
- Allows higher throughput.
- Expert results to Excel and Powerpoint
- Supplied on a USB stick
Specifications
- Optical System: Dual LED excitation (452 to 486nm and 542 to 582nm). CCD camera 4 emission filters (505 to 545nm, 562 to 596nm, 604 to 644nm, 665 to 705nm)
- Data Collection: Data collected in all four filters for all wells regardless of plate setup. Plate setup for data analysis can be altered after run completes. Melt curve analysis supports continuous data acquisition in a single filter to provide increased data point collection and reduced run times
- Installation: Plug and play design
- Precision: Discriminates 5000 and 10,000 template copies with 99% confidence
- Melt Curve: Supports continuous data acquisition in a single filter to provide increased data point collection and reduced run times
- Hertz: 50 to 60Hz
- Current: 5A
- Peak Power: 500w (typical power is 180w)
Applications
- Academic, Government and Corporate laboratories
- Human research, AgriGenomics, Translational, Forensics and many more markets
- Probe, DNA Binding Dye and HRM chemistries
- Gene expression, Viral detection, Sequencing Library
- Preparation, Genotyping and Mutation Screening applications
Warranty
1 year (parts and labor included)
Specifications
| 48-well block | |
| Validated for 5 to 20µL | |
| ±0.15°C at 60°C and 95°C | |
| 452 to 486nm and 542 to 582nm | |
| >9 logs | |
| Standard: 40 cycles in less than 40 minutes, Fast: 40 cycles in less than 20 minutes | |
| 31cm | |
| 13.6kg | |
| 120 to 240V |
| Dock and Squeegee, EcoControl and EcoStudy software USB stick, Pack of 50 Plates, Pack of 50 Plate seals, Power and Ethernet Cables | |
| 30° to 100°C | |
| ±0.1°C | |
| SYBR™, FAM™, HEX™, ROX™, Cy™5. Additional dyes within the wavelength range compatible with Pro 48 filters are supported with no additional calibration required for implementation | |
| Detection: 1 copy | |
| 34.5cm | |
| 32cm | |
| Absolute Quantification, Relative Quantification, Allelic Discrimination, High Resolution Melt (HRM) |