Search results for "pcr reagents"

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 25 X |
Description | Works on all AB platforms, but is optimized for the 7500. |
Reverse Transcriptase | ArrayScript™ |
Concentration | 25X |
PCR Method | 1-step RT-qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Gold DNA Polymerase |
Format | Tube |
Product Type | One-Step RT-PCR Reagent |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex |
Sample Type | RNA |
Reaction Speed | Standard |
Product Line | AgPath-ID™, Ambion™ |
Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
GC-Rich PCR Performance | High |
---|---|
Fidelity (vs. Taq) | 25 X |
Description | Works on all AB platforms, but is optimized for the 7500. |
Reverse Transcriptase | ArrayScript™ |
Concentration | 25X |
PCR Method | 1-step RT-qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Gold DNA Polymerase |
Format | Tube |
Product Type | One-Step RT-PCR Reagent |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 System |
Reaction Speed | Standard |
Product Line | AgPath-ID™, Ambion™ |
Thermo Scientific™ Phusion High-Fidelity PCR Kit
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Shipping Condition | Dry Ice |
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Polymerase | Phusion High-Fidelity DNA Polymerase |
GC-Rich PCR Performance | High |
Content And Storage | • Phusion DNA Polymerase (2 U/μL) • 5X Phusion GC & HF Buffer • dNTP mix (10 mM each) • Control template (λ DNA) and DNA size standard • 1.3 kb & 10 kb primers (4 μM each) • 50 mM MgCl2 solution and DMSO Store at –20°C. |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | Separate Components |
Product Type | High-Fidelity PCR Kit |
Reaction Speed | Fast |
Hot Start | No |
Overhang | Blunt |
For Use With (Application) | Standard PCR,High-fidelity PCR |
Thermo Scientific™ PCR Master Mix (2X)
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.

Polymerase | Taq DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Reaction Format | SuperMix or Master Mix |
Product Type | PCR Master Mix |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
Concentration | 2X |
Starting Material | DNA |
For Use With (Application) | Standard PCR |
Thermo Scientific™ Phusion Blood Direct PCR Kit
Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
Shipping Condition | Wet or Dry Ice |
---|---|
Polymerase | Phusion Blood II DNA Polymerase |
GC-Rich PCR Performance | High |
Reaction Format | Separate Components |
Product Type | Direct PCR Kit |
Sample Type | Blood |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2 U/μL |
Starting Material | DNA |
PCR Method | Direct PCR |
SureTect™ Listeria species PCR Assay
Rapidly detect Listeria species in food and environmental samples with the Thermo Scientific™ SureTect™ Listeria Species PCR Assay.
Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (20mL Sample prep, 50mL PCR)
Go from Biological Sample to Genotyping Results in less than one hour.
Thermo Scientific™ KingFisher™ Flex Purification System, KingFisher with 96 PCR head
Prepare samples efficiently from a variety of materials with Thermo Scientific™ KingFisher™ Flex Magnetic Particle Processors.
SuperScript™, Platinum™, SYBR™ SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit
Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.
Shipping Condition | Dry Ice |
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Polymerase | Taq DNA Polymerase |
Product Type | One-Step qRT-PCR Kit |
For Use With (Equipment) | 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ |
Sample Type | RNA |
Reaction Speed | Standard |
For Use With (Application) | Real Time PCR (qPCR) |
PCR Method | 1-step RT-qPCR |
Product Line | Platinum™, SYBR™, SuperScript™ |
Detection Method | SYBR |
Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
Shipping Condition | Wet Ice |
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Purity or Quality Grade | UP (Ultra Pure) |
Reverse Transcriptase | ArrayScript™ UP |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Gold DNA Polymerase |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | RNA |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Run Time | Standard |
Product Line | RNA-to-CT™, SYBR™ |
Applied Biosystems™ ExoSAP-IT™ Express PCR Product Cleanup Reagent
Removes excess primers and dNTPs. Does not interfere with downstream applications.

Content And Storage | -20°C |
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Thermo Scientific™ GeneJET PCR Purification Kit
Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Content And Storage | For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C. |
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Format | Spin Column |
Run Time | 5 min. |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Applied Biosystems™ ExoSAP-IT™ PCR Product Cleanup Reagent
ExoSAP-IT™ PCR Product Clean-up Reagent is used for enzymatic clean-up of amplified PCR product. It hydrolyses excess primers and nucleotides in a single step.

Shipping Condition | Dry Ice |
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Content And Storage | Shipped on dry ice. Store at -20°C. |
Protein Form | Recombinant |
Sample Type | PCR Products |
Research Category | Genetic Diseases |
For Use With (Application) | PCR Purification |
Product Line | ExoSAP-IT™ |
Thermo Scientific™ DreamTaq PCR Master Mixes (2X)
Thermo Scientific DreamTaq PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl2, and dNTPs.

Shipping Condition | Dry Ice |
---|---|
Polymerase | DreamTaq DNA Polymerase |
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Reaction Format | SuperMix or Master Mix |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
For Use With (Application) | Standard PCR |
Thermo Scientific™ Phire Plant Direct PCR Master Mix
Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

Shipping Condition | Wet or Dry Ice |
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Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Reaction Format | SuperMix or Master Mix |
Product Type | Direct PCR Master Mix |
Sample Type | Plant |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
Starting Material | DNA |
For Use With (Application) | Genotyping,Hot-start PCR |
PCR Method | Direct PCR |