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Pepsin, Extra Pure, Powder, SLR, Fisher Chemical™
Enzyme that breaks down proteins into smaller peptides.
Cytiva NAP™ Columns, NAP-10
Use for rapid and efficient purification of DNA and oligonucleotides (>10-mer) in less than 15 minutes by gravity flow. Cytiva Life Sciences™ illustra™ NAP™ Columns are disposable columns prepacked with Sephadex™ G-25 DNA Grade
Includes | Complete step-by-step protocol identifying the fixed volumes needed for equilibration, washing and sample elution |
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Product Type | NAP-10 |
For Use With (Application) | Rapid and efficient purification of DNA and Oligonucleotides |
Thermo Scientific™ RNase A, DNase and protease-free (10 mg/mL)
Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A.
Thermo Scientific™ T4 DNA Ligase (5 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Product Type | T4 DNA Ligase |
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Enzyme | DNA Ligase |
Compatible Buffer | 10X T4 DNA Ligase Buffer |
Concentration | 5 U/μL |
Thermo Scientific™ Proteinase K, recombinant, PCR grade
Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.
Form | Liquid |
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Product Type | Proteinase K |
Thermo Scientific™ RiboLock RNase Inhibitor (40 U/μL)
RiboLock RNase Inhibitor specifically inhibits the activity of RNases A, B, and C, protecting RNA under a variety of reaction conditions.
Product Type | RNase Inhibitor |
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Compatible Buffer | Storage Buffer |
Concentration | 40 U/μL |
Product Line | RiboLock™ |
TaqMan® TaqMan™ Gene Expression Assay (FAM)
Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.
Shipping Condition | Room Temperature |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 60 X |
Product Type | Gene Expression Assay |
Reaction Speed | Fast |
Green Features | Sustainable packaging |
PCR Method | qPCR |
Product Line | TaqMan™ |
Detection Method | Primer-probe |
Thermo Scientific™ Eco31I (BsaI) (10 U/μL)
The Eco31I (BsaI) restriction enzyme recognizes GGTCTC(1/5)^ sites and cuts best at 37°C in G buffer (Isoschizomers: BsaI, Bso31I, BspTNI).
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Eco31I (BsaI) |
Methylation Sensitivity | CpG Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dam Methylation-Sensitive |
Research Category | Scarless Cloning |
Compatible Buffer | 10X Buffer G |
Type IIS RE | Yes |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.
Shipping Condition | Dry Ice |
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Content And Storage | • RevertAid Reverse Transcriptase (200 U/μL) |
Reverse Transcriptase | RevertAid |
Final Product Type | First-Strand cDNA |
Concentration | 200 U/μL |
Format | Stand-alone Enzyme |
Size (Final Product) | Up to 13 kb |
Reaction Format | Separate components |
Reaction Speed | 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)
Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .
Product Type | Alkaline Phosphatase |
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Enzyme | Phosphatase |
Compatible Buffer | PCR Buffer, Enzyme Buffer |
Concentration | 1 U/μL |
Thermo Scientific™ EcoRI (10 U/μL)
The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer.
Concentration | 10 U/μL |
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Thermo Scientific™ T4 Polynucleotide Kinase (10 U/μL)
T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA.
Product Type | T4 Polynucleotide Kinase |
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Enzyme | T4 Polynucleotide Kinase |
Compatible Buffer | 10X Reaction Buffer |
Concentration | 10 U/μL |
Thermo Scientific™ FastDigest XhoI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Xho I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
First Strand cDNA Synthesis Kit
Thermo Scientific™ Fermentas First Strand cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates.
Thermo Scientific™ BcuI (SpeI) (10 U/μL)
Cut at A^CTAGT sites with BcuI (SpeI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AhlI, SpeI).
Product Type | Restriction Enzyme |
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Enzyme | BcuI (SpeI) |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer Tango |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |