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Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
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Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 25 X |
Description | Works on all AB platforms, but is optimized for the 7500. |
Reverse Transcriptase | ArrayScript™ |
Concentration | 25X |
PCR Method | 1-step RT-qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Gold DNA Polymerase |
Format | Tube |
Product Type | One-Step RT-PCR Reagent |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex |
Sample Type | RNA |
Reaction Speed | Standard |
Product Line | AgPath-ID™, Ambion™ |
Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
GC-Rich PCR Performance | High |
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Fidelity (vs. Taq) | 25 X |
Description | Works on all AB platforms, but is optimized for the 7500. |
Reverse Transcriptase | ArrayScript™ |
Concentration | 25X |
PCR Method | 1-step RT-qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Gold DNA Polymerase |
Format | Tube |
Product Type | One-Step RT-PCR Reagent |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 System |
Reaction Speed | Standard |
Product Line | AgPath-ID™, Ambion™ |
Thermo Scientific™ CloneJET PCR Cloning Kit
Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
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Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
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Format | Kit |
Sample Type | PCR Products |
Cloning Method | Restriction Enzyme⁄ MCS, Blunt PCR |
For Use With (Application) | PCR Cloning |
Vector | pJET1.2/blunt |
Product Line | CloneJET |
Thermo Scientific™ CloneJET™ PCR Cloning Kit with DH10B Competent Cells
An advanced positive-selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Any blunt or sticky-end DNA fragment can be cloned.
Content And Storage | Dry Ice (-80°C) |
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Description | Bacterial/Yeast Strain: DH10B |
For Use With (Application) | Cloning (Blunt PCR, Restriction Enzyme ⁄ MCS) |
BRAND™ PCR 12-Strip Caps
Manufactured from polypropyle without lubricants or chemical additives that can contaminate reagents and samples.PCR 12-Strip Caps are autoclavable and RNase-, DNase-, and DNA-free. Caps are domed and available in a variety of colors for sample identification.
Shape | Domed |
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Product Type | Tube Strip Cap |
Dnase Free | Yes |
For Use With (Equipment) | PCR tubes |
RNase Free | Yes |
Material | Polypropylene |
Autoclavable | Autoclavable |
Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up, kit for gel extraction or PCR clean up
Clean up PCR reactions and gel extractions with a two-in-one approach. Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit is optimized for recovery and elution volume when purifying DNA fragments via both applications.
Format | Mini Spin Column |
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Efficiency | Typical recovery: 70–95% |
Size | 50 bp to ∼20 kbp |
Isolation Technology | Silica Membrane Technology |
Color | pH Indicator: Yellow = correct conditions Green = pH slightly high (adjustment recommended) Blue = pH too high (adjustment necessary) |
Purity | A260/A280: 1.8–1.9 |
Quantity | 1 Kit |
Yield | Up to ∼15μg DNA |
Test Time | 10 min/6 preps |
Applied Biosystems™ ExoSAP-IT™ PCR Product Cleanup Reagent
ExoSAP-IT™ PCR Product Clean-up Reagent is used for enzymatic clean-up of amplified PCR product. It hydrolyses excess primers and nucleotides in a single step.
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Shipping Condition | Dry Ice |
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Content And Storage | Shipped on dry ice. Store at -20°C. |
Protein Form | Recombinant |
Sample Type | PCR Products |
Research Category | Genetic Diseases |
For Use With (Application) | PCR Purification |
Product Line | ExoSAP-IT™ |
Applied Biosystems™ ExoSAP-IT™ Express PCR Product Cleanup Reagent
Removes excess primers and dNTPs. Does not interfere with downstream applications.
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Content And Storage | -20°C |
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Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up XS Columns for gel extraction and PCR clean up
Columns for concentrated clean up with small elution volumes
Applied Biosystems™ HT ExoSAP-IT™ Fast High-Throughput PCR Product Cleanup Reagent
Removes excess primers and dNTPs. Does not interfere with downstream applications. Ideal for high-throughput PCR product cleanup using liquid handling platforms and multi-channel pipettes.
Content And Storage | Store at −20°C. Stable for one month at 4°C |
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Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe.
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GC-Rich PCR Performance | Low |
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Format | Tube(s) |
Internal Probe Modification | TAMRA™ Quencher (3'), VIC™ (5') |
Product Type | Exogenous Internal Positive Control Reagent |
Reaction Speed | Standard |
Green Features | Sustainable packaging |
PCR Method | qPCR |
Product Line | TaqMan™ |
BRAND™ PCR 12-Strip Tubes
Manufactured from polypropylene without lubricants or chemical additives that can contaminate reagents and samples.PCR 12-Strip Tubes are autoclavable and RNase-, DNase-, and DNA-free. Thin, uniform walls to ensure consistent, precise thermal transfer for excellent results. Available with or without caps and can be ordered separately.
Format | Strip Plate |
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Dnase Free | Yes |
Total Volume (Metric) Well | 0.2 mL |
RNase Free | Yes |
Material | Polypropylene |
Certifications/Compliance | Certified RNase/DNase-free |
Autoclavable | Autoclavable |
Corning™ Molecular Biology Reagents
SureTRACE
Supports traceability with guaranteed access to certificates and proactive change notifications.
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Supports traceability with guaranteed access to certificates and proactive change notifications.
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Used in molecular biology, nucleic acid purification applications
Applied Biosystems™ TaqMan™ RNase P Detection Reagents Kit
TaqMan™ RNase P Detection Reagents Kit