Buffers and Standards
Buffers and Standards
Hydrogen ion buffers comprising a mixture of a weak acid and its conjugate base or vice-versa and used to stabilize pH; also diluents, washing solutions, and standard-value solutions used for a broad array of scientific calibration purposes.
The buffers used to calibrate pH meters may be certified and/or traceable to the National Institute of Standards and Technology (NIST). These buffers may also be color-coded for easy identification:
- Red: pH 4.0
- Yellow: pH 7.0
- Blue: pH 10.0
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SERVA Tris-Tricine/SDS Electrophoresis Buffer (10x), Serva Electrophoresis™
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™
Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT
Name Note | 50X Solution |
---|---|
Concentration or Composition (by Analyte or Components) | Tris (24%), Acetic Acid (5.0%), and EDTA (<2%). |
CAS | 6850-28-8 |
Protease | Protease free |
Physical Form | Liquid |
Grade | Electrophoresis |
Synonym | TAE |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
DNase | DNase free |
Recommended Storage | RT |
Filtered Through | Filtered through a 0.2-micron filter. |
ChemAlert Storage Symbol | Gray |
TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™
CAS: 10043-35-3 Synonym: TBE
CAS | 10043-35-3 |
---|---|
Synonym | TBE |
TAE Buffer, Tris-Acetate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents
Name Note | 10X Solution |
---|---|
Concentration or Composition (by Analyte or Components) | Tris (<5.0%), Acetic Acid (1.0%), and EDTA (<1%). |
CAS | 77-86-1 |
Protease | Protease free |
Physical Form | Liquid |
Chemical Name or Material | Tris-Acetate-EDTA |
Grade | Electrophoresis |
Synonym | TAE |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
DNase | DNase free |
Recommended Storage | RT |
Filtered Through | Filtered through a 0.2-micron filter. |
ChemAlert Storage Symbol | Gray |
TBE Buffer, Tris-Borate-EDTA, 1X Solution, Electrophoresis, Fisher BioReagents
Name Note | 1X Solution |
---|---|
CAS | 7732-18-5 |
Protease | Protease free |
Color | Colorless |
Physical Form | Liquid |
pH | 8.2 to 8.4 |
Chemical Name or Material | Tris-Borate-EDTA |
Grade | Electrophoresis |
Synonym | TBE |
DNase | DNase free |
Recommended Storage | RT |
Filtered Through | Filtered through a 0.2-micron filter. |
ChemAlert Storage Symbol | Gray |
Tris-Borate-Edta Buffer, for Electrophoresis, (10X Concentration), Fisher Chemical™
CAS: 610769-35-2 Molecular Formula: C14H30BN3O14 Molecular Weight (g/mol): 475.211 MDL Number: 4679 InChI Key: OSBLTNPMIGYQGY-UHFFFAOYSA-N PubChem CID: 21257725 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;boric acid SMILES: B(O)(O)O.C(CN(CC(=O)O)CC(=O)O)N(CC(=O)O)CC(=O)O.C(C(CO)(CO)N)O
PubChem CID | 21257725 |
---|---|
CAS | 610769-35-2 |
Molecular Weight (g/mol) | 475.211 |
MDL Number | 4679 |
SMILES | B(O)(O)O.C(CN(CC(=O)O)CC(=O)O)N(CC(=O)O)CC(=O)O.C(C(CO)(CO)N)O |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;boric acid |
InChI Key | OSBLTNPMIGYQGY-UHFFFAOYSA-N |
Molecular Formula | C14H30BN3O14 |
Color | Undesignated |
---|---|
Physical Form | Liquid |
Chemical Name or Material | Tris-Glycine-SDS |
Grade | Electrophoresis |
Identification | Pass Test |
DNase | DNase free |
Filtered Through | Filtered through a 0.2-micron filter. |
ChemAlert Storage Symbol | Gray |
Name Note | 10X Solution |
Concentration or Composition (by Analyte or Components) | 0.25M Tris Base, 1.92M Glycine, and 1.0% (w/v) SDS. |
CAS | 151-21-3 |
Protease | Protease free |
Synonym | TG-SDS |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
Recommended Storage | RT |
Thermo Scientific Chemicals BICINE, Electrophoresis Grade, 99+%
CAS: 150-25-4 Molecular Formula: C6H12NNaO4 Molecular Weight (g/mol): 185.16 MDL Number: MFCD00004295 InChI Key: MFBDBXAVPLFMNJ-UHFFFAOYSA-M Synonym: N,N-Bis(2-hydroxyethyl)glycine PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]acetic acid SMILES: [Na+].OCCN(CCO)CC([O-])=O
PubChem CID | 8761 |
---|---|
CAS | 150-25-4 |
Molecular Weight (g/mol) | 185.16 |
ChEBI | CHEBI:40957 |
MDL Number | MFCD00004295 |
SMILES | [Na+].OCCN(CCO)CC([O-])=O |
Synonym | N,N-Bis(2-hydroxyethyl)glycine |
IUPAC Name | 2-[bis(2-hydroxyethyl)amino]acetic acid |
InChI Key | MFBDBXAVPLFMNJ-UHFFFAOYSA-M |
Molecular Formula | C6H12NNaO4 |
Color | Undesignated |
---|---|
Physical Form | Liquid |
Chemical Name or Material | Tris-Glycine |
Grade | Electrophoresis |
DNase | DNase free |
Filtered Through | Filtered through a 0.2-micron filter. |
ChemAlert Storage Symbol | Gray |
Name Note | 10X Solution |
Concentration or Composition (by Analyte or Components) | Components: Tris (2-3%) and Glycine (13-14%). 0.25M Tris Base and 1.92M Glycine. |
CAS | 56-40-6 |
Protease | Protease free |
Synonym | TG |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
Recommended Storage | RT |
Tricine, Electrophoresis Grade, 99%, Thermo Scientific Chemicals
CAS: 5704-04-1 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 MDL Number: MFCD00004277 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N Synonym: N-(Trishydroxymethylmethyl)glycine PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO
PubChem CID | 79784 |
---|---|
CAS | 5704-04-1 |
Molecular Weight (g/mol) | 179.17 |
ChEBI | CHEBI:39063 |
MDL Number | MFCD00004277 |
SMILES | C(C(=O)O)NC(CO)(CO)CO |
Synonym | N-(Trishydroxymethylmethyl)glycine |
IUPAC Name | 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid |
InChI Key | SEQKRHFRPICQDD-UHFFFAOYSA-N |
Molecular Formula | C6H13NO5 |
Thermo Scientific Chemicals Tris(hydroxymethyl)aminomethane, Electrophoresis Grade, 99.5%
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: TRIS PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
PubChem CID | 6503 |
---|---|
CAS | 77-86-1 |
Molecular Weight (g/mol) | 121.136 |
ChEBI | CHEBI:9754 |
MDL Number | MFCD00004679 |
SMILES | C(C(CO)(CO)N)O |
Synonym | TRIS |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
Molecular Formula | C4H11NO3 |
Thermo Scientific™ Tris(hydroxymethyl)aminomethane, Electrophoresis Grade, 99.9%
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: 2-Amino-2-hydroxymethyl-1,3-propanediol; TRIS PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
PubChem CID | 6503 |
---|---|
CAS | 77-86-1 |
Molecular Weight (g/mol) | 121.136 |
ChEBI | CHEBI:9754 |
MDL Number | MFCD00004679 |
SMILES | C(C(CO)(CO)N)O |
Synonym | 2-Amino-2-hydroxymethyl-1,3-propanediol; TRIS |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
Molecular Formula | C4H11NO3 |
Tricine, Electrophoresis Grade, MP Biomedicals
CAS: 5704-04-1 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO
PubChem CID | 79784 |
---|---|
CAS | 5704-04-1 |
Molecular Weight (g/mol) | 179.17 |
ChEBI | CHEBI:39063 |
SMILES | C(C(=O)O)NC(CO)(CO)CO |
IUPAC Name | 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid |
InChI Key | SEQKRHFRPICQDD-UHFFFAOYSA-N |
Molecular Formula | C6H13NO5 |
Thermo Scientific Chemicals TRIS-glycine native sample buffer (4X), pH 8.6
For preparation of protein samples for native gel electrophoresis
Color | Blue |
---|---|
Physical Form | Liquid |
pH | 8.6 |
Chemical Name or Material | Sample Loading Buffer |
Concentration | 4X |
For Use With (Application) | Preparation of protein samples for native gel electrophoresis |