Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

1 – 15 of 253 results

TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents

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Specifications

Color	Colorless
Physical Form	Liquid
Chemical Name or Material	Tris-EDTA
Grade	Molecular Biology
DNase	DNase free
Filtered Through	Filtered through a 5-micron filter.
ChemAlert Storage Symbol	Gray
Name Note	1X Solution, pH 8.0
CAS	7732-18-5
Protease	Protease free
Health Hazard 3	Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0
Health Hazard 2	CAUTION!
pH	7.4 to 8.1
Synonym	TE
Recommended Storage	RT

Phosphate Buffered Saline (PBS), 10X Powder, pH 7.4, Fisher BioReagents™

Phosphate Buffered Saline (PBS), 10X Powder provides a convenient way to make 1 L of 10X stock PBS solution.

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Thermo Scientific Chemicals Phosphate-buffered saline (PBS, 10X), pH 7.4

Non-toxic to most cells and is used in biological research.

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Specifications

Dilution	10 X
Form	Liquid
Product Type	Phosphate Buffered Saline (PBS)
Color	Colorless
pH	7.4

Thermo Scientific Chemicals PIPES, 1.0M buffer soln., pH 6.8

CAS: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight (g/mol): 302.36 MDL Number: MFCD00006159 InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N PubChem CID: 79723 ChEBI: CHEBI:44933 IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

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Specifications

PubChem CID	79723
CAS	5625-37-6
Molecular Weight (g/mol)	302.36
ChEBI	CHEBI:44933
MDL Number	MFCD00006159
SMILES	C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
IUPAC Name	2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
InChI Key	IHPYMWDTONKSCO-UHFFFAOYSA-N
Molecular Formula	C8H18N2O6S2

HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents™

Commonly used buffering agent

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Specifications

Color	Undesignated
Physical Form	Liquid
Chemical Name or Material	HEPES Buffer
Grade	Molecular Biology
Identification	Pass Test
DNase	DNase free
Merck Index	15, 4689
Concentration	0.95 to 1.05 M
ChemAlert Storage Symbol	Gray
Absorbance	≤0.01(0.1M solution) at 260nm,0.004 max. (0.1M solution) at 280nm
Name Note	1M Solution, pH 7.3
CAS	7732-18-5
Protease	Protease free
pH	7.2 to 7.5
Synonym	N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid
Purity Grade Notes	DNase-, RNase- and Protease-Free
Recommended Storage	RT
Molecular Formula	C₈H₁₈N₂O₄S
Formula Weight	238.197

Thermo Scientific Chemicals MES, 0.5M buffer soln., pH 6.0

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 MDL Number: MFCD00283848 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O

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PubChem CID	16218417
CAS	145224-94-8
Molecular Weight (g/mol)	213.248
MDL Number	MFCD00283848
SMILES	C1COCCN1CCS(=O)(=O)O.O
IUPAC Name	2-morpholin-4-ylethanesulfonic acid;hydrate
InChI Key	MIIIXQJBDGSIKL-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

Thermo Scientific Chemicals Glycine, 0.2M buffer soln., pH 2.5

CAS: 56-40-6 Molecular Formula: C2H5NO2 Molecular Weight (g/mol): 75.07 MDL Number: MFCD00008131 InChI Key: DHMQDGOQFOQNFH-UHFFFAOYSA-N IUPAC Name: 2-aminoacetic acid SMILES: NCC(O)=O

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Specifications

CAS	56-40-6
Molecular Weight (g/mol)	75.07
MDL Number	MFCD00008131
SMILES	NCC(O)=O
IUPAC Name	2-aminoacetic acid
InChI Key	DHMQDGOQFOQNFH-UHFFFAOYSA-N
Molecular Formula	C2H5NO2

Thermo Scientific Chemicals HEPES, 1.0M buffer soln., pH 7.5

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

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PubChem CID	23831
CAS	7365-45-9
Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
MDL Number	MFCD00006158
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Molecular Formula	C8H18N2O4S

Thermo Scientific Chemicals TE buffer, (20X), pH 7.4, autoclaved

CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 MDL Number: MFCD00236359 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl

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PubChem CID	93573
CAS	1185-53-1
Molecular Weight (g/mol)	157.594
MDL Number	MFCD00236359
SMILES	C(C(CO)(CO)N)O.Cl
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride
InChI Key	QKNYBSVHEMOAJP-UHFFFAOYSA-N
Molecular Formula	C4H12ClNO3

Thermo Scientific Chemicals HEPES, 1.0M buffer soln., pH 8.0

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Specifications

PubChem CID	23831
CAS	7365-45-9
Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
MDL Number	MFCD00006158
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
IUPAC Name	2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Molecular Formula	C8H18N2O4S

Thermo Scientific Chemicals TRIS-buffered saline (TBS, 10X) pH 7.4

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00132476 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

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PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00132476
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 7.4

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Thermo Scientific Chemicals TRIS-buffered saline (TBS, 10X) pH 7.4, for Western blot

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

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Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

TBS, Tris Buffered Saline, 10X Solution, pH 7.4, Molecular Biology, Fisher BioReagents™

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Specifications

Boiling Point	100°C
Color	Colorless
Physical Form	Liquid
Chemical Name or Material	Tris Buffered Saline
Grade	Molecular Biology
DNase	DNase free
ChemAlert Storage Symbol	Gray
Name Note	10X Solution
Concentration or Composition (by Analyte or Components)	10X solution contains 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.25M Tris/Tris-HCl.
CAS	7732-18-5
Protease	Protease free
pH	7.5
Synonym	TBS
Purity Grade Notes	DNase-, RNase- and Protease-Free
Recommended Storage	RT

Thermo Scientific Chemicals HEPES-buffered saline, pH 7.0 (5X)

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Specifications

PubChem CID	23831
CAS	7365-45-9
Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
MDL Number	MFCD00006158
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
IUPAC Name	2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Molecular Formula	C8H18N2O4S

Life Science Buffers

Life Science Buffers

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