HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.

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Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns

Use these endcapped, ultrapure, silica-based columns to generate exceptional peak shape and resolution for HPLC and LC/MS analyses using generic gradients

Pricing and Availability

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Thermo Scientific™ Hypersil™ ODS C18 Columns

Use as a general purpose C18 phase for acidic and neutral analytes.

Pricing and Availability
Specifications

Pore Size	120 Å
Carbon Load	10%
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
pH	2 to 8
Surface Area	170 m²/g
USP Type	L1

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Thermo Scientific™ Hypersil™ BDS C18 Columns

Use as a robust, general-purpose column for a wide range of applications in QC/QA labs.

Pricing and Availability
Specifications

Carbon Load	11%
Stationary Phase	C18
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
Surface Area	170 m²/g
USP Type	L1

Thermo Scientific™ Hypersil™ PREP HS C18 HPLC Columns

Maximize retention and loading with Hypersil PREP HS C18 prep LC reversed phase columns which offers high loading capacity and suitable for USP-L1 Applications.

Pricing and Availability
Specifications

Pore Size	100 Å
Carbon Load	15%
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Packing Material	Spherical, Fully Porous, Ultrapure Silica
Column Type	Reversed Phase
pH	2 to 9
Surface Area	350 m²/g
USP Type	L1

Thermo Scientific™ Acclaim™ HILIC-10 HPLC Columns

Use this HILIC column to retain and separate highly polar molecules that are unretained by reversed-phase chromatography.

Pricing and Availability
Specifications

Pore Size	120 Å
Carbon Load	8%
Stationary Phase	Proprietary
Temperature	70°C
Phase	HILIC
For Use With	LC/MS
Endcapped	Yes
Column Type	HILIC
pH	2 to 8
Surface Area	300 m²/g
Product Line	Acclaim

Thermo Scientific™ Acclaim™ 300 C18 HPLC Columns

Use these wide-pore C18 columns to achieve high-resolution protein separations.

Pricing and Availability
Specifications

Pore Size	300 Å
Particle Size	3 μm
Carbon Load	8%
Stationary Phase	C18
Temperature	60°C
For Use With	LC/MS
Endcapped	Yes
Column Type	Reversed Phase
pH	2.5 to 7.5
Green Features	Responsibly packaged
Surface Area	100 m²/g
Product Line	Acclaim
USP Type	L1

Hamilton™ PRP-X100 Anion Exchange HPLC Columns: Stainless Steel

Polymeric column packing for separation of inorganic and organic anions from 10 to 500 ppm

Pricing and Availability
Specifications

Pore Size	100 Å
Type	Column
Stationary Phase	PRP-X100
Temperature	5°C to 60°C
Phase	Ion Exchange
Packing Material	PSDVB/Trimethylammonium
Column Type	Ion Exchange
Mobile Phase Compatibility	pH 1 to 13, 0 to 100% aqueous, organic modifier
pH	1 to 13
Material	Stainless Steel
Max. Pressure	5000 psi (345 bar)
USP Type	L47

Thermo Scientific™ MAbPac™ SCX-10 HPLC Columns

Use these strong cation-exchange columns for high-resolution, high efficiency analysis of monoclonal antibodies (mAbs) and associated variants.

Pricing and Availability
Specifications

Pore Size	Nonporous
Temperature	Ambient to 60°C
Phase	Ion Exchange
Column Type	Ion Exchange
pH	2 to 12

Thermo Scientific™ HyperREZ™ XP Carbohydrate H+ HPLC Columns

Manufactured with a proprietary process and subjected to stringent quality control procedures, these LC Columns are used for the appropriate counter-ion to meet application requirements.

Pricing and Availability
Specifications

Particle Size	8 μm
Phase	Ligand Exchange
For Use With	LC/MS
Packing Material	Spherical, Microporous Polymer
pH	1 to 11
Max. Pressure	725 psi (50 bar)

Thermo Scientific™ Hypersil GOLD™ C8 HPLC Columns

Have analytes with medium hydrophobicity or need a less hydrophobic phase to obtain optimum retention? These columns provide outstanding sensitivity and excellent peak shape.

Pricing and Availability
Specifications

Pore Size	175 Å
Carbon Load	8%
Stationary Phase	C8
Temperature	60°C
Column Type	Reversed Phase
pH	2 to 9
Surface Area	220 m²/g
USP Type	L7

Macherey-Nagel™ Nucleosil™ 100-5 Nautilus Analytical HPLC Standard EC Column

Pricing and Availability
Specifications

Pore Size	100 Å
Solvents	Acetonitrile/water
For Use With	HPLC
Endcapped	Yes
Quantity	1 Ea.
Crosslinking	Octadecyl
Column Chemistry	Silica
Column Format	Analytical Column
Particle Size	5 μm
Type	Analytical EC Column
Carbon Load	16%
Packing Material	Silica
Mobile Phase Compatibility	Acetonitrile/water
pH	2 to 8

Thermo Scientific™ Hypersil™ PREP ODS C18 HPLC Columns

Perfect for routine workflows, our Hypersil PREP ODS preparative LC column is a general purpose C18 used in a broad range of USP L1 applications.

Pricing and Availability
Specifications

Pore Size	130 Å
Carbon Load	10%
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Packing Material	Spherical, Fully Porous Silica
Column Type	Reversed Phase
pH	2 to 8
Surface Area	195 m²/g
USP Type	L1

Thermo Scientific™ Hypersil GOLD™ SAX HPLC Columns

Analyze smaller organic molecules, including nucleotides and organic acids, quickly and efficiently with Thermo Scientific™ Hypersil™ GOLD SAX LC Columns.

Pricing and Availability
Specifications

Pore Size	175 Å
Carbon Load	2.5%
Stationary Phase	Quaternary Amine
Temperature	60°C
Phase	Ion Exchange
Column Type	Ion Exchange
pH	2 to 8
Surface Area	220 m²/g
USP Type	L14

Thermo Scientific™ BioBasic™ SCX HPLC Columns

BioBasic SCX LC Columns are optimized for the separation of proteins, peptides and other cationic species.

Pricing and Availability
Specifications

Pore Size	300 Å
Particle Size	5 μm
Carbon Load	3%
Stationary Phase	Sulfonic Acid
Temperature	60°C
Phase	Ion Exchange
Endcapped	No
Column Type	Ion Exchange
pH	2 to 8
Surface Area	100 m²/g
Max. Pressure	5800 psi (400 bar)
Product Line	BioBasic
USP Type	L52

Thermo Scientific™ BioBasic™ 8 HPLC Columns

Use this column for reversed-phase separation of proteins and other biomolecules.

Pricing and Availability
Specifications

Pore Size	300 Å
Particle Size	5 μm
Carbon Load	5%
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
pH	2 to 9
Surface Area	100 m²/g
Max. Pressure	5800 psi (400 bar)
Product Line	BioBasic
USP Type	L7

HPLC Columns

HPLC Columns

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Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns SureTRACESupports traceability with guaranteed access to certificates and proactive change notifications.Learn More

Thermo Scientific™ MAbPac™ SCX-10 HPLC Columns SureTRACESupports traceability with guaranteed access to certificates and proactive change notifications.Learn More

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Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns

Thermo Scientific™ MAbPac™ SCX-10 HPLC Columns