Chemical reagents, kits, and other products used for DNA sequencing; includes sequencing and pre-sequencing kits, purification kits, buffers, standards, etc.
The Invitrogen Collibri PCR-free PS DNA Library Preparation kits are designed for next-generation sequencing (NGS) on Illumina systems. The kit supports whole-genome sequencing (WGS) applications and is suitable for all genomes, including FFPE samples.
The PCR Product Pre-Sequencing Kit uses a novel, enzymatic clean-up method to pre-treat PCR products prior to sequencing, without any subsequent purification or separation steps.
Our fastest PCR clean-up method - just 5 minutes to superior sequencing results. Now available with optional tracking dye. It is necessary to remove excess primers and unincorporated nucleotides from the PCR reaction prior to sequencing.
The DNA contained in the preparation has been lyophilized to maximize stability.The two hydrolytic enzymes used in this kit, shrimp alkaline phosphatase and exonuclease I, effectively remove excess dNTPs and primers.
The matrix standard set DS-32 is used to generate the 'multicomponent matrix' required when analyzing 5-FAM™, JOE™, NED™, and ROX™ labeled DNA fragments on the Applied Biosystems™ 31xx, 3500 and 3730 Series Systems.