Modifying Enzymes

Modifying Enzymes
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Thermo Scientificâ„¢ RNase A, DNase and protease-free (10 mg/mL)
Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A.
Thermo Scientific™ T4 DNA Ligase (5 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Product Type | T4 DNA Ligase |
---|---|
Enzyme | DNA Ligase |
Compatible Buffer | 10X T4 DNA Ligase Buffer |
Concentration | 5 U/μL |
Thermo Scientific™ DNase I, RNase-free (1 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Content And Storage | -20°C |
---|---|
Form | Solid |
Product Type | Proteinase K |
Color | White |
For Use With (Application) | Isolation of DNA and RNA from cell and tissue preparations, inactivating many Enzymes, including Endogenous Nucleases |
Source | Tritirachium album |
Thermo Scientific™ FastAP Thermosensitive Alkaline Phosphatase (1 U/μL)
Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .
Product Type | Alkaline Phosphatase |
---|---|
Enzyme | Phosphatase |
Compatible Buffer | PCR Buffer, Enzyme Buffer |
Concentration | 1 U/μL |
Thermo Scientificâ„¢ RNase A/T1 Mix
Get the RNA degradation activity of both RNase A and RNase T1 with Thermo Scientificâ„¢ RNase A/T1 Mix.
Thermo Scientific™ RNase T1 (1000 U/μL)
RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues.
Product Type | RNase T1 |
---|---|
Enzyme | RNase |
Concentration | 1000 U/μL |
Thermo Scientific™ DNase I, RNase-free, HC (50 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Thermo Scientificâ„¢ Proteinase K, recombinant, PCR grade
Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.
Form | Liquid |
---|---|
Product Type | Proteinase K |
Thermo Scientific™ T4 DNA Ligase, HC (30 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Thermo Scientific™ RNase I (10 U/μL)
Ribonuclease I (RNase I) is an endoribonuclease that hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates.
Product Type | RNase I |
---|---|
Enzyme | RNase |
Concentration | 10 U/μL |
Thermo Scientific™ RNase H (5 U/μL)
Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids.
Product Type | RNase H |
---|---|
Enzyme | RNase |
Compatible Buffer | 10X Reaction Buffer |
Concentration | 5 U/μL |
Thermo Scientific™ Terminal Deoxynucleotidyl Transferase (20 U/μL)
Terminal Deoxynucleotidyl Transferase (TdT) catalyzes the template-independent addition of deoxyribonucleotides to the 3'-OH terminus of DNA molecules.
Product Type | TdT |
---|---|
Enzyme | Terminal Deoxynucleotidyl Transferase |
Compatible Buffer | Storage Buffer, 5X Reaction Buffer |
Concentration | 20 U/μL |
Thermo Scientificâ„¢ CpG Methyltransferase (M.SssI)
Get fast reaction times without compromising reaction efficiency with Thermo Scientificâ„¢ CpG Methyltransferase (M.SssI), one of the basic tools used in epigenetic studies.
MP Biomedicalsâ„¢ Proteinase K from Tritirachium album
Highly active stable endopeptidase with a broad spectrum of action
Content And Storage | 4°C |
---|---|
Inhibitors | Diisopropylfluorophosphate or Phenylmethane Sulfonyl Fluoride, AEBSF and Trypsin |
Form | Lyophilized Powder |
Product Type | Proteinase K |
Molecular Weight (g/mol) | 30000 |
Color | White |
pH | 7.5 to 12 |
Grade | Molecular Biology |
For Use With (Application) | Useful for the proteolytic inactivation of Nucleases during the isolation of DNA and RNA |
Source | Tritirachium album |