
Thermo Scientific™ FastDigest HindIII
The FastDigest HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Hind III |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest EcoRI
The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | EcoR I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ BpiI (BbsI) (10 U/μL)
Cut GAAGAC(2/6)^ sites with BpiI (BbsI) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: BbsI, BpuAI, BstV2I).

Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | BpiI (BbsI) |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Scarless Cloning |
Compatible Buffer | 10X Buffer G |
Type IIS RE | Yes |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/ÎĽL |
Thermo Scientific™ FastDigest PvuI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Thermo Scientific™ FastDigest BamHI
Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | BamH I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ NTP Set, 100 mM Solution
The Thermo Scientific NTP set is a convenient set of 100 mM solutions of each ATP, CTP, GTP and UTP.

Thermo Scientific™ FastDigest BglII
Cut at A^GATCT sites with FastDigest™ BglII restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Sensitive to Heat Inactivation | No |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Bgl II |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Product Line | FastDigest™ |
Thermo Scientific™ XbaI (10 U/μL)
Cut at T^CTAGA sites with XbaI restriction enzyme, which functions best at 37°C in Tango buffer.

Concentration | 10 U/ÎĽL |
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Thermo Scientific™ FastDigest SgsI
Cut at GG^CGCGCC sites with Thermo Scientific™ FastDigest™ AscI (SgsI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PalAI).

Thermo Scientific™ NotI (10 U/μL)
The NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in O buffer (Isoschizomers: CciNI).

Concentration | 10 U/ÎĽL |
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Thermo Scientific™ BcuI (SpeI) (10 U/μL)
Cut at A^CTAGT sites with BcuI (SpeI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AhlI, SpeI).

Product Type | Restriction Enzyme |
---|---|
Enzyme | BcuI (SpeI) |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer Tango |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/ÎĽL |
Thermo Scientific™ NdeI (10 U/μL)
The NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in O buffer (Isoschizomers: FauNDI).

Concentration | 10 U/ÎĽL |
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Thermo Scientific™ Eco32I (EcoRV) (10 U/μL)
The Eco32I (EcoRV) restriction enzyme recognizes GAT^ATC sites and cuts best at 37°C in R buffer (Isoschizomers: EcoRV).

Concentration | 10 U/ÎĽL |
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Thermo Scientific™ Cfr42I (SacII) (10 U/μL)
The Cfr42I (SacII) restriction enzyme recognizes CCGC^GG sites and cuts best at 37°C in B buffer (Isoschizomers: KspI, SacII, Sfr303I, SgrBI, SstII).

Concentration | 10 U/ÎĽL |
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Thermo Scientific™ HindIII (10 U/μL)
The HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer.

Concentration | 10 U/ÎĽL |
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