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Thermo Scientific™ CloneJET PCR Cloning Kit
Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
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Format | Kit |
Sample Type | PCR Products |
Cloning Method | Restriction Enzyme⁄ MCS, Blunt PCR |
For Use With (Application) | PCR Cloning |
Vector | pJET1.2/blunt |
Product Line | CloneJET |
Thermo Scientific™ Lambda DNA
Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.
Thermo Scientific™ pUC19 DNA
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Invitrogen™ Collibri™ Library Quantification Kit
Invitrogen™ Collibri™ Library Quantification Kit includes a ready-to-use master mix optimized for Illumina NGS library quantification and a library dilution buffer
Shipping Condition | Dry Ice |
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Polymerase | Platinum™ II Taq Hot Start DNA Polymerase |
GC-Rich PCR Performance | Low |
Content And Storage | Store at -20°C. |
Format | Kit |
Product Type | Library Quantification Kit |
For Use With (Equipment) | 7500 Fast System, 7900HT Fast System, QuantStudio™, ViiA™ 7 System, StepOne™ System, StepOnePlus™ System |
Sample Type | DNA,RNA |
PCR Method | qPCR |
For Use With (Application) | Illumina NGS Library Quantification |
Test Time | Standard |
Product Line | Collibri™ |
Detection Method | SYBR |
Thermo Scientific™ Rapid DNA Ligation Kit
Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.
Product Type | Rapid DNA Ligation Kit |
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Enzyme | DNA Ligase |
Thermo Scientific™ pBR322 Plasmid DNA
Optimize cloning with Thermo Scientific™ pBR322 DNA, a 4361 bp double-stranded closed circular medium copy plasmid DNA.
Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit
The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
Format | 12-well Strips,8-well Strip,96- or 384-well Plate |
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Product Type | Sequencing Buffer |
For Use With (Equipment) | SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers |
Technique | Fluorescent Dye Terminator Sequencing |
Product Line | BigDye™ Terminator |
Applied Biosystems™ Sanger Sequencing Kit
Sanger Sequencing Kit includes all reagents needed for sample preparation for sanger sequencing
Thermo Scientific™ pUC18 DNA
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Applied Biosystems™ BigDye™ Direct Cycle Sequencing Kit
The BigDye™ Direct Cycle Sequencing Kit, part of the BigDye™ product family, is a complete kit for your PCR and Sequencing needs.
Shipping Condition | Dry Ice |
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Format | 8-well Strip,96-well Plate |
Product Type | Direct Cycle Sequencing Kit |
Technique | Fluorescent Dye Terminator Sequencing |
For Use With (Application) | Comparative Sequencing (Germline Mutations - 30:70 Heterozygotes), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, Standard Sequencing, Gap Closure, Homopolymer A or T, AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations -10:90 Heterozygotes) |
Template Compatibility | BAC DNA, Cosmids, Lambda DNA, PCR Amplicons (10:90 Heterozygotes), Plasmid DNA (≤15Kb), Genomic DNA (Bacterial), Fosmids, PCR Amplicons (50:50 Heterozygotes), PCR Amplicons (30:70 Heterozygotes) |
Product Line | BigDye™ |
Thermo Scientific™ CloneJET™ PCR Cloning Kit with DH10B Competent Cells
An advanced positive-selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Any blunt or sticky-end DNA fragment can be cloned.
Content And Storage | Dry Ice (-80°C) |
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Description | Bacterial/Yeast Strain: DH10B |
For Use With (Application) | Cloning (Blunt PCR, Restriction Enzyme ⁄ MCS) |
Applied Biosystems™ POP-7™ Polymer for 3730/3730xl DNA Analyzers
POP-7™ Polymer is a separation matrix for the 3730 and 3730xl DNA Analyzers. POP-7™ Polymer is customized for DNA sequencing and fragment analysis.
Accuracy | 98.5% up to 900 bases (for 50 cm capillary), 98.5% up to 700 bases (for 36 cm capillary) |
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Product Type | Polymer |
For Use With (Application) | Standard Sequencing, Fragment Analysis (Denaturing) |
Test Time | 1 hr for 36 cm capillary (std. seq.), 2 hrs for 50 cm capillary (long read seq.) |
Product Line | POP-7™ |
Applied Biosystems™ BigDye™ Terminator v1.1 Cycle Sequencing Kit
This BigDye™ Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.
Product Type | Sequencing Buffer |
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For Use With (Equipment) | SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐Well and 384‐Well Thermal Cyclers |
Technique | Fluorescent Dye Terminator Sequencing |
Template Compatibility | BAC DNA, Plasmid DNA (≤15 Kb), PCR Amplicons, Single Stranded DNA |
Product Line | BigDye™ Terminator |
Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit
The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry
Thermo Scientific™ Maxima H Minus Double-Stranded cDNA Synthesis Kit
The Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA.
Reverse Transcriptase | Maxima H Minus |
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Reaction Format | Separate Components |
Final Product Type | Double Stranded cDNA |
Product Type | cDNA Synthesis Kit |
Sample Type | RNA |
For Use With (Application) | Cloning |
Product Line | Maxima |