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Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Thermo Scientific™ NP-40 Surfact-Amps™ Detergent Solution
Thermo Scientific™ Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized and supplied as a 10% solution.
Form | Liquid |
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Reagent Type | Detergent Solution |
Product Line | Surfact-Amps™ |
Cytiva Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophobic
Excellent for coupling oligos or proteins directly to the beads. Their cauliflower-like structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds.
Content And Storage | 2°C to 8°C |
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Diameter (Metric) | 1 μm |
Concentration | 5% solids (50mg/mL) |
For Use With (Application) | DNA Sample Prep,Protein Purification |
Thermo Scientific™ Aprotinin Protease Inhibitor
Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds.
Cytiva PD MiniTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Cytiva Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophilic
Excellent for coupling oligos or proteins directly to the beads. Their cauliflower-like structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds.
Content And Storage | 2°C to 8°C |
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Diameter (Metric) | 1 μm |
Concentration | 5% solids (50mg/mL) |
For Use With (Application) | DNA Sample Prep,Protein Purification |
Thermo Scientific™ High Select™ Phosphopeptide Enrichment Kits & Reagents
The expanded High Select family of complete kits supports enrichment of phosphorylated peptides using Fe-NTA and TiO2 immobilized resins in spin column, tip, and magnetic bead formats designed for use with MS analysis.
Cytiva HisTrap™ FF Columns: 16 x 25mm
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
Flow Rate | <20 mL/min. |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Diameter (English) Inner | 0.6 in. |
Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Diameter (Metric) Inner | 16 mm |
Material | Polypropylene |
Support Type | 6% Cross-linked Agarose |
Cytiva PreScission™ Protease
Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. Cytiva Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.
Particle Size | 75μm |
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Buffer | Aqueous Buffer |
Ligand Type | N-benzyl-n-methyl Ethanolamine |
Height (Metric) | 25mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 12 |
Product Type | HiTrap Capto adhere column |
Column Type | Affinity Column |
Material | Polypropylene |
For Use With (Application) | Easy screening and convenient process development |
Support Type | Highly Cross-linked Agarose |
Height (English) | 0.98 in. |
Cytiva SpeedBeads™ Magnetic Neutravidin Coated Particles, 1 um, 1% solids, 0.05% Na azide
Capture of Biotinylated Probes. Their cauliflower-like structure gives Neutravidin beads high binding capacity. Neutravidin SpeedBeads combine fast reaction kinetics and low, non-specific binding for increased throughput and precision in immunoassay and molecular biology applications.
Includes | 0.05% Na azide |
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Content And Storage | 2°C to 8°C |
Diameter (Metric) | 1 μm |
Concentration | 1% solids (10mg/mL) |
For Use With (Application) | Immunoassays,cDNA Library Construction,Capture of Biotinylated Targets |
Thermo Scientific™ Lysozyme
Degrade unwanted bacterial cell wall in protein or nucleic acid extracts
Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit
Chromogenic Endotoxin Quant Kits are highly sensitive endpoint assays that measure and detect endotoxin (LPS) in protein, peptide, nucleic acid, or antibody samples using the Amebocyte Lysate Assay from 0.01–1.0 EU/mL. Kits are available in reaction sizes ranging from 30 to 240.
Shipping Condition | Wet Ice |
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Assay | End-point Chromogenic Assay |
Type | Endotoxin Quantitation Kit |
Target | Endotoxins |
Content And Storage | • Lyophilized amebocyte lysate • E.coli endotoxin standard • Chromogenic substrate • Endotoxin-free water Store at 2–8°C upon receipt. |
Format | Kit |
Assay Sensitivity | 0.01 EU/mL |
Assay Range | 0.01 to 0.1 EU/mL, 0.1 to 1.0 EU/mL |
Product Line | Pierce™ |
Detection Method | Colorimetric |
Thermo Scientific™ HisPur™ Ni-NTA Resin
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Stationary Phase | Divalent Nickel, Metal Chelate |
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Type | Resin |
Target | His-Tagged Protein |
Content And Storage | Store at 4°C |
Format | Bottle |
Description | HisPur Ni-NTA Resin |
Form | Liquid Suspension |
Column Type | Affinity, Agarose Resin |
Capacity (Metric) | Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin |
Product Line | HisPur™ |
Thermo Scientific™ T-PER™ Tissue Protein Extraction Reagent
Extract total protein from tissue samples using this mild cell lysis and extraction reagent developed specifically for tissues.