Test
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
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Format | Kit |
Sample Type | PCR Products |
Cloning Method | Restriction Enzyme⁄ MCS, Blunt PCR |
For Use With (Application) | PCR Cloning |
Vector | pJET1.2/blunt |
Product Line | CloneJET |
Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.
Product Type | Rapid DNA Ligation Kit |
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Enzyme | DNA Ligase |
Perform cloning, sequencing of insert DNA, and generation of nested deletions with ExoIII using this double-stranded closed circular high copy plasmid DNA.
Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.
Perform DNA cloning, dideoxy DNA sequencing, in vitro mutagenesis, and in vitro transcription in one system with this double-stranded closed circular high copy phagemid DNA.
Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.
Perform blunting and phosphorylation of DNA ends in just 5 minutes for subsequent use in blunt-end ligation with this kit.
An advanced positive-selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Any blunt or sticky-end DNA fragment can be cloned.
Content And Storage | Dry Ice (-80°C) |
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Description | Bacterial/Yeast Strain: DH10B |
For Use With (Application) | Cloning (Blunt PCR, Restriction Enzyme ⁄ MCS) |
Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.
A versatile and efficient tool for introducing point mutations, insertions, or deletions into any type of plasmid DNA. With this kit the entire plasmid is amplified using phosphorylated primers that introduce the desired changes.
Prepare DNA molecular weight standards with Thermo Scientific™ phiX174 RF1 DNA, a 5386 bp double-stranded closed circular plasmid.
Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.