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Optimal Reaction Temperature

30°C (9)
37°C (443)
50°C (2)
55°C (21)
65°C (25)

Sensitive to Heat Inactivation

No (66)
Yes (433)

Methylation Sensitivity

CpG Methylation-Sensitive (239)
Dam Methylation-Sensitive (37)
Dcm Methylation-Sensitive (22)
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Not Dam Methylation-Sensitive (456)
Not Dcm Methylation-Sensitive (443)

Reaction Speed

15 to 30 min. (3)
15 to 30 sec./kb (8)
30 min. (16)
60 min. (9)
8 hr. (1)
9 hr. (1)
Fast (101)
Fast or Standard (79)
Standard (193)

GC-Rich PCR Performance

High (245)
Low (65)
Medium (21)

1 – 15 of 816 results

Applied Biosystems™ TaqMan™ Universal PCR Master Mix

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

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Specifications

Real Time PCR Master Mixes

Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Product Type	Universal PCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Product Line	TaqMan™
Detection Method	Primer-probe

SYBR™ Power SYBR™ Green PCR Master Mix

Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

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Specifications

Real Time PCR Master Mixes

GC-Rich PCR Performance	High
Purity or Quality Grade	LD (Low DNA)
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Detection Method	SYBR
Polymerase	AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
Label or Dye	SYBR Green
Product Line	SYBR™

Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG

TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.

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Specifications

Real Time PCR Master Mixes

Shipping Condition	Wet Ice
Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Product Type	Universal PCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Standard
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Product Line	TaqMan™
Detection Method	Primer-probe

Applied Biosystems™ SNaPshot™ Multiplex Kit

The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).

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Specifications

Direct PCR Reagents and Kits

Product Type	Multiplex Kit
For Use With (Equipment)	3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers
Sample Type	DNA
Technique	SNP Genotyping (Fragment Analysis-Based), Primer Extension
Product Line	SNaPshot™
Detection Method	Primer-probe

Applied Biosystems™ SYBR™ Green Universal Master Mix

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

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Specifications

Real Time PCR Master Mixes

Polymerase	AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance	High
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
Reaction Speed	Standard
Label or Dye	SYBR Green
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	SYBR™
Detection Method	SYBR

Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)

Human GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)

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Real Time PCR Assays and Arrays

Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)

Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)

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Real Time PCR Assays and Arrays

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

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Pricing and Availability
Specifications

Real Time PCR Master Mixes

Shipping Condition	Wet Ice
Polymerase	Dual-Lock Taq DNA Polymerase
Format	Tube
Product Type	Real Time PCR SYBR Master Mix
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type	DNA
Reaction Speed	Fast or Standard
Concentration	2X
For Use With (Application)	Gene Expression
PCR Method	qPCR
Product Line	PowerUP™, SYBR™
Detection Method	SYBR

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.

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Specifications

High Fidelity PCR Reagents and Kits

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Content And Storage	• Phusion DNA Polymerase (2 U/μL) • 5X Phusion HF & GC Buffers • DMSO • 50 mM MgCl₂ solution Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl₂ in the final 1X concentration. Store at -20°C.
Fidelity (vs. Taq)	52X
Size (Final Product)	20 kb or less
Reaction Format	Standalone
Product Type	High-Fidelity DNA Polymerase
Reaction Speed	Fast
Hot Start	No
Overhang	Blunt
Concentration	2 U/μL
For Use With (Application)	Standard PCR,High-fidelity PCR

Thermo Scientific™ DNase I, RNase-free (1 U/μL)

Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.

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Modifying Enzymes

Thermo Scientific™ DreamTaq Green PCR Master Mix (2X)

Thermo Scientific DreamTaq Green PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq Green buffer, MgCl₂, and dNTPs.

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Specifications

Standard PCR Reagents and Kits

Shipping Condition	Dry Ice
Polymerase	DreamTaq DNA Polymerase
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Reaction Format	SuperMix or Master Mix
Reaction Speed	Standard
Hot Start	No
Overhang	3'-A
Concentration	2X
For Use With (Application)	Standard PCR

Thermo Scientific™ RNase A, DNase and protease-free (10 mg/mL)

Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A.

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Modifying Enzymes

Thermo Scientific™ TAE Buffer (Tris-acetate-EDTA) (50X)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).

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Restriction Enzymes

Thermo Scientific™ dNTP Mix (10 mM each)

Thermo Scientific dNTP Mix (10 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.

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Specifications

dNTPs

Shipping Condition	Dry Ice
Purity or Quality Grade	>99%
Form	Liquid
Label or Dye	Unlabeled
Concentration	10 mM

Thermo Scientific™ Bovine Serum Albumin (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin.

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Restriction Enzymes

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Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited) Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited) Greener Choice ProductThis product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More About the Greener Choice Program

Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)

Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)