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Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Cytiva Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophobic
Excellent for coupling oligos or proteins directly to the beads. Their cauliflower-like structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds.
Content And Storage | 2°C to 8°C |
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Diameter (Metric) | 1 μm |
Concentration | 5% solids (50mg/mL) |
For Use With (Application) | DNA Sample Prep,Protein Purification |
Cytiva PD MiniTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Cytiva Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophilic
Excellent for coupling oligos or proteins directly to the beads. Their cauliflower-like structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds.
Content And Storage | 2°C to 8°C |
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Diameter (Metric) | 1 μm |
Concentration | 5% solids (50mg/mL) |
For Use With (Application) | DNA Sample Prep,Protein Purification |
Cytiva HisTrap™ FF Columns: 16 x 25mm
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
Flow Rate | <20 mL/min. |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Diameter (English) Inner | 0.6 in. |
Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Diameter (Metric) Inner | 16 mm |
Material | Polypropylene |
Support Type | 6% Cross-linked Agarose |
Cytiva PreScission™ Protease
Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. Cytiva Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.
Particle Size | 75μm |
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Buffer | Aqueous Buffer |
Ligand Type | N-benzyl-n-methyl Ethanolamine |
Height (Metric) | 25mm |
Content And Storage | 4° to 30°C |
pH Range | 3 to 12 |
Product Type | HiTrap Capto adhere column |
Column Type | Affinity Column |
Material | Polypropylene |
For Use With (Application) | Easy screening and convenient process development |
Support Type | Highly Cross-linked Agarose |
Height (English) | 0.98 in. |
Cytiva SpeedBeads™ Magnetic Neutravidin Coated Particles, 1 um, 1% solids, 0.05% Na azide
Capture of Biotinylated Probes. Their cauliflower-like structure gives Neutravidin beads high binding capacity. Neutravidin SpeedBeads combine fast reaction kinetics and low, non-specific binding for increased throughput and precision in immunoassay and molecular biology applications.
Includes | 0.05% Na azide |
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Content And Storage | 2°C to 8°C |
Diameter (Metric) | 1 μm |
Concentration | 1% solids (10mg/mL) |
For Use With (Application) | Immunoassays,cDNA Library Construction,Capture of Biotinylated Targets |
Cytiva Superose™ 6 Increase Columns
High-resolution size-exclusion chromatography for studying large proteins and complexes. Cytiva Lifescience™ Superose™ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC).
Particle Size | 8.6μm |
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Ligand Type | Cross-linked agarose |
Content And Storage | 4° to 30° in 20% ethanol |
pH Range | 3 to 12 |
Column Type | Gel Filtration Column |
Material | Borosilicate glass |
Max. Pressure | 435 psi |
Cytiva His GraviTrap™ Columns
Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.
Cytiva HisTrap™ FF Crude Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Particle Size | 45 to 165 μm |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Cross-linked Agarose |
Cytiva Gel Filtration Calibration Kits
Calibrate gel filtration columns simply, reliably. Cytiva Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards.
Content And Storage | 2° to 8°C |
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Product Type | Calibration Kit |
For Use With (Equipment) | High-resolution media and prepacked column |
Quantity | Each |
For Use With (Application) | Calibration of high-resolution gel filtration media and column such as Superdex™, Superose and Sephacryl |
Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns
For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.
Cytiva HisTrap™ FF Columns: 5 x 5mL
Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.