Test
Stabilize enzymes and other proteins which possess free sulfhydryl groups with DTT, shown to restore activity lost by oxidation of these groups in vitro.
Product Type | DTT (Dithiothreitol) |
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Concentration | 0.1 M |
Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
Product Type | X-Gal |
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Optimize biochemistry or molecular biology applications that require a chelator for divalent cations.
Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X Orange Loading Dye.
Prepare RiboRuler RNA ladders and RNA samples for electrophoresis on agarose or polyacrylamide gels using Thermo Scientific™ 2X RNA Loading Dye.
Thermo Scientific 25 mM MgCl2 is an aqueous solution of 0.22 μm membrane-filtered magnesium chloride, used for optimization of magnesium ion concentration in PCR.
Effectively screen colonies with X-Gluc, a substrate for ß-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene.
Substrate Properties | Chemical Substrate |
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Product Type | X-Gluc |
Substrate Type | Beta-Glucuronidase (GUS) Substrate |
Detection Method | Colorimetric |
Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X TriTrack DNA Loading Dye.
Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.
Perform agarose gel analysis of DNA samples that contain high amounts of DNA binding proteins with this DNA loading dye containing SDS.
Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.
Precipitate DNA and RNA with this 0.22μm membrane-filtered, sterile, nuclease-free solution of sodium acetate.
Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
Detect expression of the URA3 gene, which encodes orotine-5'-monophosphate (OMP) dicarboxylase with Thermo Scientific™ 5-Fluoroorotic Acid, used in yeast molecular genetics.
Form | Powder |
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Product Type | 5-Fluoroorotic Acid |
Chemical Name or Material | 5-Fluoroorotic Acid |
Firefly luciferase substrate for in vitro and in vivo applications
Substrate Properties | Chemical Substrate |
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Content And Storage | Store at or below -20°C. |
Substrate Type | Luciferase Substrate |
For Use With (Application) | ATP Assay, Gene Reporter Assay |
Product Line | Pierce™ |
Detection Method | Bioluminescence |