PCR Equipment and Supplies
PCR Equipment and Supplies
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Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG
TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.
Shipping Condition | Wet Ice |
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Polymerase | AmpliTaq Gold DNA Polymerase |
GC-Rich PCR Performance | High |
Product Type | Universal PCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
PCR Method | qPCR |
Product Line | TaqMan™ |
Detection Method | Primer-probe |
Applied Biosystems™ SYBR™ Green Universal Master Mix
Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.
Polymerase | AmpliTaq Gold DNA Polymerase |
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GC-Rich PCR Performance | High |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | SYBR™ |
Detection Method | SYBR |
Cytiva PuReTaq Ready-To-Go™ PCR Beads
Pre-mixed, pre-dispensed reaction beads optimized for performing standard PCR amplifications. Cytiva Lifescience™ illustra™ PuReTaq Ready-To-Go™ PCR Beads are stable at ambient temperature.
Content And Storage | Room temperature stable beads containing ∼ 2.5 units of recombinant PuReTaq DNA Polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction Buffer, When a bead is reconstituted to a 25μL final volume, the concentration of each dNTP |
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Quantity | 1 Kit |
For Use With (Application) | Standard PCR |
Applied Biosystems™ TaqMan™ Universal PCR Master Mix
TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.
Polymerase | AmpliTaq Gold DNA Polymerase |
---|---|
GC-Rich PCR Performance | High |
Product Type | Universal PCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
PCR Method | qPCR |
Product Line | TaqMan™ |
Detection Method | Primer-probe |
Applied Biosystems™ MycoSEQ™ Mycoplasma Detection Kits
The MycoSEQ Mycoplasma Detection Kit is a real-time mycoplasma PCR assay designed and validated to meet the requirements of European Pharmacopeia Chapter 2.6.7 for use as a nucleic acid amplification technique (NAT).
Description | Detection for BioProduction process media, cells, raw materials |
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Label or Dye | SYBR Green |
Quantity | 100 Reactions |
For Use With (Application) | Mycoplasma Detection |
Product Line | MycoSEQ™ |
Applied Biosystems™ Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Eukaryotic 18S rRNA Endogenous Control (VIC™/MGB probe, primer limited)
Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
Shipping Condition | Wet Ice |
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Polymerase | Dual-Lock Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | PowerUP™, SYBR™ |
Detection Method | SYBR |
Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | Standalone |
Product Type | High-Fidelity DNA Polymerase |
Reaction Speed | Fast |
Hot Start | No |
Overhang | Blunt |
Concentration | 2 U/μL |
For Use With (Application) | Standard PCR,High-fidelity PCR |
Thermo Scientific™ dNTP Mix (10 mM each)
Thermo Scientific dNTP Mix (10 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.
Shipping Condition | Dry Ice |
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Purity or Quality Grade | >99% |
Form | Liquid |
Label or Dye | Unlabeled |
Concentration | 10 mM |
Thermo Scientific™ DreamTaq PCR Master Mixes (2X)
Thermo Scientific DreamTaq PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl2, and dNTPs.
Shipping Condition | Dry Ice |
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Polymerase | DreamTaq DNA Polymerase |
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Reaction Format | SuperMix or Master Mix |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
For Use With (Application) | Standard PCR |
Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit
Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.
Shipping Condition | Dry Ice |
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Content And Storage | • RevertAid Reverse Transcriptase |
Reverse Transcriptase | RevertAid |
Final Product Type | First-Strand cDNA |
For Use With (Application) | RT-PCR |
Format | Kit |
Size (Final Product) | Up to 13 kb |
Reaction Format | Separate components |
Reaction Speed | 60 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
TaqMan® TaqMan™ Gene Expression Assay (FAM)
Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.
Shipping Condition | Room Temperature |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 60 X |
Product Type | Gene Expression Assay |
Reaction Speed | Fast |
Green Features | Sustainable packaging |
PCR Method | qPCR |
Product Line | TaqMan™ |
Detection Method | Primer-probe |
Thermo Scientific™ GeneJET PCR Purification Kit
Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
Format | Spin Column |
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Run Time | 5 min. |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Thermo Scientific™ Oligo(dT)18 Primer
Thermo Scientific Oligo(dT)18 Primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends.
Shipping Condition | Dry Ice |
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Primer Length | 18-mer |
Form | Liquid |
Product Type | Primer |
Primer | Oligo dT |
Concentration | 0.5 μg/μL |
For Use With (Application) | cDNA Synthesis |
Thermo Scientific™ dNTP Set, 100 mM Solutions
Thermo Scientific dNTP Set is a convenient set of 100 mM aqueous solutions of each of dATP, dCTP, dGTP and dTTP, supplied in separate vials.
Shipping Condition | Dry Ice |
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Purity or Quality Grade | >99% |
Form | Liquid |
Label or Dye | Unlabeled |
Concentration | 100 mM |
For Use With (Application) | Standard PCR,High-fidelity PCR,Real Time PCR (qPCR) |