Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

1 – 15 of 562 results

Thermo Scientific Chemicals HEPES, 99%

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

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Specifications

PubChem CID	23831
CAS	7365-45-9
Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
MDL Number	MFCD00006158
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
Synonym	4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
IUPAC Name	2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Molecular Formula	C8H18N2O4S

Phosphate Buffered Saline (PBS), 10X Powder, pH 7.4, Fisher BioReagents™

Phosphate Buffered Saline (PBS), 10X Powder provides a convenient way to make 1 L of 10X stock PBS solution.

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PBS Tablets, Phosphate Buffered Saline, Fisher BioReagents™

Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!

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PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™

Commonly used buffer

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Specifications

Content And Storage	Room temperature
Form	Liquid

Thermo Scientific Chemicals MES monohydrate, 98%

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 MDL Number: MFCD00149409 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O

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Specifications

PubChem CID	16218417
CAS	145224-94-8
Molecular Weight (g/mol)	213.248
MDL Number	MFCD00149409
SMILES	C1COCCN1CCS(=O)(=O)O.O
IUPAC Name	2-morpholin-4-ylethanesulfonic acid;hydrate
InChI Key	MIIIXQJBDGSIKL-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents

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Specifications

Color	Colorless
Physical Form	Liquid
Chemical Name or Material	Tris-EDTA
Grade	Molecular Biology
DNase	DNase free
Filtered Through	Filtered through a 5-micron filter.
ChemAlert Storage Symbol	Gray
Name Note	1X Solution, pH 8.0
CAS	7732-18-5
Protease	Protease free
Health Hazard 3	Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0
Health Hazard 2	CAUTION!
pH	7.4 to 8.1
Synonym	TE
Recommended Storage	RT

Thermo Scientific Chemicals MES monohydrate, 99+%, for biochemistry

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 195.23 MDL Number: MFCD00149409 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N Synonym: 2-(N-Morpholino)ethanesulfonic acid monohydrate PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O

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Specifications

PubChem CID	16218417
CAS	145224-94-8
Molecular Weight (g/mol)	195.23
MDL Number	MFCD00149409
SMILES	C1COCCN1CCS(=O)(=O)O.O
Synonym	2-(N-Morpholino)ethanesulfonic acid monohydrate
IUPAC Name	2-morpholin-4-ylethanesulfonic acid;hydrate
InChI Key	MIIIXQJBDGSIKL-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

Invitrogen™ NuPAGE™ MES SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled

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Specifications

Formulation	1X: 50 mM MES, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA, pH 7.3
Physical Form	Liquid
pH	7.3
Chemical Name or Material	Running Buffer
Concentration	20X
Product Line	NuPAGE

Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

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Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
SMILES	C(C(CO)(CO)N)O
Synonym	Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™

Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT

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Specifications

Name Note	50X Solution
Concentration or Composition (by Analyte or Components)	Tris (24%), Acetic Acid (5.0%), and EDTA (<2%).
CAS	6850-28-8
Protease	Protease free
Physical Form	Liquid
Grade	Electrophoresis
Synonym	TAE
Purity Grade Notes	DNase-, RNase- and Protease-Free
DNase	DNase free
Recommended Storage	RT
Filtered Through	Filtered through a 0.2-micron filter.
ChemAlert Storage Symbol	Gray

Tris-HCl, 1M Solution, pH 8.0, Molecular Biology Grade, Ultrapure, Thermo Scientific Chemicals

CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl

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Specifications

PubChem CID	93573
CAS	1185-53-1
Molecular Weight (g/mol)	157.594
SMILES	C(C(CO)(CO)N)O.Cl
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride
InChI Key	QKNYBSVHEMOAJP-UHFFFAOYSA-N
Molecular Formula	C4H12ClNO3

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™

Commonly used buffering agent.

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Specifications

Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
Color	White
Chemical Name or Material	HEPES
Grade	Molecular Biology
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
Identification	Pass Test
DNase	DNase free
Merck Index	15, 4689
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
ChemAlert Storage Symbol	Gray
Assay Percent Range	≥99 %
PubChem CID	23831
Absorbance	0.01 max. (0.1M solution) at 280nm
Percent Purity	≥99%
CAS	7365-45-9
Protease	Protease free
Health Hazard 3	Emergency Overview Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. . NFPA Health:2 Flammability:1 Instability:1
MDL Number	MFCD00006158
Health Hazard 2	WARNING!
Solubility Information	Soluble in water
pH	5.0 to 6.5
Purity Grade Notes	DNase-, RNase- and Protease-Free
Recommended Storage	RT
Molecular Formula	C8H18N2O4S

Corning™ Phosphate Buffered Saline, 1X

Used alone or in combination with other agents for washing tissues and cells.

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Thermo Scientific™ Pierce™ Concentrated Buffer Stocks (10X and 20X)

Save time using Pierce Concentrated Buffer stock solutions (10X and 20X) with simple dilutions to expedite your experiment without sacrificing quality or consistency.

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Thermo Scientific Chemicals Phosphate-buffered saline (PBS, 1X), sterile-filtered

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Life Science Buffers

Life Science Buffers

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Corning™ Phosphate Buffered Saline, 1X SureTRACESupports traceability with guaranteed access to certificates and proactive change notifications.Learn More

Corning™ Phosphate Buffered Saline, 1X