Optimize Your Immunoprecipitation (IP) with ChromoTek Nano-Traps

Ready-to-Use Affinity Beads for Fast and Efficient Immunoprecipitation

ChromoTek Nano-Traps are the benchmark in immunoprecipitation (IP) and allow fast and reliable single-band pulldown of low expressed proteins. They consist of nanobodies coupled to beads and are ready to use in the following applications: IP, Co-IP, Co-IP/mass spectrometry (MS), on-bead assay, ChIP/RIP analysis, and split-fluorescent protein assay.

Browse all Chromotek Nano-Traps

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Nano-Trap Benefits

The benefiits of the Nano-Trap system include:

• No heavy and light antibody chains
• Stringent washing conditions
• Low host-cell protein background
• Very efficient pulldown, with no protein in flow-through
• High affinity to binding of proteins expressed at low levels
• Short incubation time (30-60 min)
• Recombinantly expressed and validated

Available Nano-Trap Targets

Examples of Nano-Trap targets include those listed below and many more:

• GFP
• V5
• DYKDDDDK
• Myc
• HA-tag
• Ubiquitin

When using Nano-Traps, the amount of immunoprecipitated protein of interest (POI) is significantly higher and the background is reduced in contrast to an IP conducted with a conventional antibody (See Figure 1). Nano-Traps provide pure fractions of immunoprecipitated POI without contamination of heavy and light antibody chains.

Figure 1: IP of a protein of interest (POI) by Nano-Traps (left) compared with conventional antibodies coupled to Protein A/G beads (right), analyzed by SDS-PAGE. M: marker; B: bound.

GFP-Traps for Immunoprecipitation

When using a GFP-Trap™ for pull-down of GFP-fusion proteins, the amount of immunoprecipitated GFP is significantly higher and the background is reduced in contrast to an IP conducted with a conventional anti-GFP antibody conjugated to Protein A/G beads.

No Heavy and Light Chain Antibody Fragments, and Superior Background

Heavy and light antibody chains are contaminating; however, a GFP-Trap provides pure fractions of immunoprecipitated GFP-fusion protein without such contamination (see Figure 2). ChromoTek GFP-Traps have been cited by researchers over 5,000 times and are the most frequently cited monoclonal anti-GFP antibody. As such, they are the gold standard for immunoprecipitation of GFP-fusion protein.

Figure 2: IP of GFP using a GFP-Trap compared with a conventional anti-GFP antibody coupled to Protein A/G beads, analyzed by SDS-PAGE. B: bound; I: input; FT: flow-through.

Ready-to-Use GFP-Trap Formats

GFP-Traps consists of an anti-GFP nanobody conjugated to different resins. The GFP-Trap is available as agarose beads, magnetic agarose beads, magnetic particles, or 96-well plates. Examples of these GFP-Traps and their features/uses are given below:

• GFP-Trap Agarose: Lowest background and high binding capacity IP
• GFP-Trap Magnetic Agarose: Magnetic separation and high binding capacity IP
• GFP-Trap Magnetic Particles (M-270): Pull-down of large proteins/complexes
• GFP-Trap Multiwell Plates: High-throughput applications and ELISA
• GFP-Trap Kits: Including lysis buffer for mammalian cells, wash, and elution buffers