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BD Biosciences™ 488 EdU Click Proliferation Kit (RUO)
For analyzing DNA synthesis in cycling cells.
Brand: BD Biosciences™ 565455
This item is not returnable.
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Description
- Provides a tool for analyzing DNA synthesis in cycling cells.
- In this method, the thymidine analog EdU (5-ethynyl-2´-deoxyuridine) is incorporated into newly synthesized DNA by cells progressing through the S (DNA synthesis) phase of the cell cycle.
- EdU can then be detected in fixed and permeabilized cells with a fluorophore-labeled azide.
- The reaction of the fluorophore-labeled azide and EdU occurs via a copper-catalyzed click reaction between the azide moiety of the fluorophore and the alkyne moiety of EdU.
- When co-stained with a DNA dye such as 7-AAD, PI, or DAPI, cell populations may be segmented by flow cytometry into the G0/G1-phases (2N DNA content, EdU-negative), S-phase (2N-4N DNA content, EdU-positive), or G2/M-phases (4N DNA content, EdU-negative).
- Contains 6-FAM Azide, which is excited by the blue laser and has an excitation maximum of 496 nm and an emission maximum of 516 nm.
Specifications
| EdU, 6-FAM Azide, Buffer Additive (10X) to be stored dry and protected from light at -20°C. Saponin-based Permeabilization and Wash Reagent (10X), Fixative, Catalyst, DMSO to be stored dry at 2 - 8°C or room temperature. | |
| 50 Tests | |
| Flow Cytometry, Fluorophore | |
| 10 mg EdU, 130 μL 6-FAM Azide, 400 mg Buffer Additive (10X), 50 mL Saponin-based Permeabilization and Wash Reagent (10X), 5 mL Fixative, 2 mL Catalyst, 5 mL DMSO. |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- 488 EdU Click Proliferation Kit
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Carcinogenicity Category 1A
- Serious eye damage/eye irritation Category 1
- Germ cell mutagenicity Category 2
- Skin corrosion/irritation Category 2
- Skin sensitiser Category 1
- Specific target organ toxicity Category 3
Hazard Statement
- H315-Causes skin irritation.
- H317-May cause an allergic skin reaction.
- H318-Causes serious eye damage.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
- H341-Suspected of causing genetic defects.
- H350-May cause cancer.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
- P370+P378-In case of fire: Use CO2, powder or waterspray to extinguish.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : formaldehyde, coppersulphate
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