missing translation for 'onlineSavingsMsg'
Learn More
Learn More
BD Biosciences™ 647 EdU Click Proliferation Kit (RUO)
For analyzing DNA synthesis in cycling cells.
Brand: BD Biosciences™ 565456
This item is not returnable.
View return policy
Description
- Provides a tool for analyzing DNA synthesis in cycling cells.
- In this method, the thymidine analog EdU (5-ethynyl-2´-deoxyuridine) is incorporated into newly synthesized DNA by cells progressing through the S (DNA synthesis) phase of the cell cycle.
- EdU can then be detected in fixed and permeabilized cells with a fluorophore-labeled azide.
- The reaction of the fluorophore-labeled azide and EdU occurs via a copper-catalyzed click reaction between the azide moiety of the fluorophore and the alkyne moiety of EdU.
- When co-stained with a DNA dye such as 7-AAD, PI, or DAPI, cell populations may be segmented by flow cytometry into the G0/G1-phases (2N DNA content, EdU-negative), S-phase (2N-4N DNA content, EdU-positive), or G2/M-phases (4N DNA content, EdU-negative).
- Contains Eterneon™ Red 645 Azide, which is excited by the red laser and has an excitation maximum of 643 nm and an emission maximum of 662 nm.
Specifications
| EdU, Eterneon™ Red 645 Azide, Buffer Additive (10X) to be stored dry and protected from light at -20°C. Saponin-based Permeabilization and Wash Reagent (10X), Fixative, Catalyst, DMSO to be stored dry at 2 - 8°C or room temperature. | |
| 50 Tests | |
| Flow Cytometry, Fluorophore | |
| 10 mg EdU, 130 μL Eterneon™ Red 645 azide (10 mM), 400 mg Buffer Additive (10X), 50 mL Saponin-based Permeabilization and Wash Reagent (10X), 5 mL Fixative, 2 mL Catalyst, 5 mL DMSO. |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- 647 EdU Click Proliferation Kit
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- Carcinogenicity Category 1A
- Serious eye damage/eye irritation Category 1
- Germ cell mutagenicity Category 2
- Skin corrosion/irritation Category 2
- Skin sensitiser Category 1
- Specific target organ toxicity Category 3
Hazard Statement
- H315-Causes skin irritation.
- H317-May cause an allergic skin reaction.
- H318-Causes serious eye damage.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
- H341-Suspected of causing genetic defects.
- H350-May cause cancer.
Precautionary Statement
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : formaldehyde
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Spot an opportunity for improvement?Share a Content Correction