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Abnova ES-E14TG2a (Mouse pluripotent embryonic stem cell) whole cell lysate (nondenatured)
Brand: Abnova L045V2.50ug
This item is not returnable.
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Description
- Maintained in the undifferentiated state using specialized culturing protocols
- Monitored for undifferentiated cell morphology
- Analyzed using Flow Cytometry against specific cell marker
- Coommasie Blue Staining of total cell lysate in SDS-PAGE
Western Blotting, Immunoprecipitation
Specifications
| Immunoprecipitation, Western Blot | |
| Whole cell lysate (non-denatured) | |
| Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). | |
| 50 μg | |
| Mouse | |
| Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Embryonic | |
| Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. | |
| 12.5% SDS-PAGE Stained with Coomassie Blue. | |
| In modified RIPA Lysis Buffer. | |
| 4 mg/mL |
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Product Title
Abnova ES-E14TG2a (Mouse pluripotent embryonic stem cell) whole cell lysate (nondenatured) > 50μg
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