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Cytiva Activated Thiol Sepharose™ 4B Medium
For reversible coupling of proteins to Sepharose 4B via a glutathione spacer arm
Brand: Cytiva 17-0640-01
This item is not returnable.
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Description
- Gel reacts with heavy metal ions and with alkyl and aryl halides
- Addition reactions occur with compounds containing C=O, C=C, and N=N bonds
- Less substituted but more porous than Thiopropyl Sepharose 6B
- Suitable for covalent chromatography of large molecules such as enzymes and nucleic acids
- Possible to couple proteins lacking thiol groups to thiol gels using the heterobifunctional reagent SPDP, facilitating use of covalent chromatography
Specifications
| 1μM Activated Thiol/mL Medium | |
| 90 μm | |
| 15 g | |
| Used for reversible immobilization of molecules containing Thiol groups under mild conditions, optimized for immobilization of large molecules |
| 4% Agarose | |
| 2 to 8 | |
| 2°C to 8°C | |
| 4% Agarose |