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Invitrogen™ Shrimp Alkaline Phosphatase (SAP)

Treat unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. Shrimp Alkaline Phosphatase (SAP) is useful in molecular biology applications such as the dephosphorylation of phosphorylated ends of DNA or RNA for subsequent use in cloning or end-labeling of probes.

POA

Specifications

For Use With (Application) Dephosphorylation of DNA/RNA
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Products 3
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15533687
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Invitrogen™
78390500UN
500 units
POA
500 units
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15523687
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Invitrogen™
783901000UN
1000 units
POA
1000 units
In Stock
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15543687
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Invitrogen™
783905000UN
5000 units
POA
5000 units
In Stock
to see stock.
Add to basket
 
Description

Description

  • 100% heat-inactivated in 5 min at 65°C
  • Significantly improved storage stability at lower temperatures
  • Very high specific activity (see Fig. 3)
  • Removes 5’-phosphates from DNA, RNA, dNTPs, and proteins
  • Purified from a recombinant source
  • May be added directly to restriction enzyme digests
  • No vector purification necessary
  • Requires no supplemental zinc or other additives for activity
  • Works direct in many different buffers
  • Easy treatment of unincorporated dNTPs in PCR products prior to DNA sequencing or SNP analysis
Specifications

Specifications

Dephosphorylation of DNA/RNA
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