Thermo Scientific™ alamarBlue™ Cell Viability Assay Reagent

Description

Utilize a redox-sensitive growth indicator to quantitatively measure cell viability and proliferation using fluorescent or colorimetric detection strategies with Thermo Scientific™ alamarBlue Cell Viability Assay Reagent.

Additional Information and Online Ordering

The alamarBlue Reagent is a non–toxic, water-soluble resazurin dye that yields a fluorescent signal and a colorimetric change when incubated with metabolically active cells. The reagent is designed for cell viability and proliferation studies using mammalian cell lines, bacteria and fungi and is ideally suited for in vitro cytotoxicity studies. Additionally, cell viability can be monitored in real time when performing a variety of tissue engineering applications.

Highlights:

• High sensitivity and linearity – reproducibly detect as few as 50 cells

• Quantitative – provides accurate measurement over time

• Non-toxic to cells – less likely to interfere with normal metabolism

• Scalable – mix–and–read, homogeneous assay enhances speed while minimizing effort

• No cell lysis – ideal for use with time course experiments or post–measurement functional assays

• Stable – allows for continuous cell growth monitoring over a long period

• Flexible – can be used with primary cell lines, adherent or suspension cells, bacteria and fungi

• Versatile – allows fluorescent or colorimetric detection methods

Requires:

Fluorescent plate reader for excitation at 530 to 560nm and emission detection at 590nm, or spectrophotometer plate reader capable of reading 570nm and 600nm absorbance.

Recommended for:

• Monitor cell proliferation and cell viability for in vitro cytotoxicity studies (e.g., effects of environmental toxins, cytokines, drugs, siRNA/microRNA)
• Monitor cell viability in real time for 3D tissue engineering applications