Invitrogen™ Alexa Fluor™ Plus 555 Phalloidin

Description

• Alexa Fluor Plus 555 Phalloidin is a high-affinity filamentous actin (F-actin) probe (phalloidin) conjugated to our bright, photostable, orange/TRITC fluorescent Alexa Fluor Plus 555 dye. For the best possible sensitivity and specificity, Alexa Fluor Plus 555 Phalloidin is synthesized to maximize the units of Alexa Fluor Plus 555 Phalloidin per length of F-actin.
•  Selectively stains F-actin with 3–4 times the sensitivity of Alexa Fluor 555 Phalloidin
• Outstanding fluorescence performance for sharp actin cytoskeleton images
• Excitation/emission: 555/565 nm. Compatible with standard TRITC/RFP filter set or laser settings
• More sensitive and specific than antibody staining against actin
• Optimal for fixed and permeabilized samples
• Phalloidin is a bi-cyclic peptide belonging to a family of toxins originating from the deadly Amanita phalloides 'death cap' mushroom and commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor Plus 555 dye provides orange/TRITC fluorescence of unparalleled brightness and photostability.
• Alexa Fluor Plus 555 Phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Its staining is fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot nanocrystals, and other Alexa Fluor conjugates including secondary antibodies.