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Cytiva Amersham™ AlkPhos Direct™ ECF Detection Module
Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes
Brand: Cytiva RPN3685
This item is not returnable.
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Description
AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star™ or ECF™ detection can be used. DNA or RNA probes are labeled during a 30-minute reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration.
- Hybridization stringency can be controlled by elevating temperatures up to 75°C
- Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
- ECF detection for quantitation experiments
- Rapid protocol saves two to three hours over conventional indirect methods
Order Info
Each system include the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500cm2: labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, ECF detection reagents.
Specifications
| 2500cm2 membrane | |
| Sufficient for 25 or 50 labeling reactions and detection of 2500cm2 membrane: labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, ECF detection reagents. | |
| Each |
Safety and Handling
Signal Word
Hazard Category
- Danger
Hazard Category
- Serious eye damage/eye irritation Category 1
- Skin corrosion/irritation Category 2
- Specific target organ toxicity after repeated exposure Category 2