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Cytiva Amersham™ AlkPhos Direct™ Labeling Modules
Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes. AlkPhos Direct Labeling Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase.
Brand: Cytiva RPN3681
This item is not returnable.
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Description
DNA or RNA probes are labeled during a 30-minute reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration.- Hybridization stringency can be controlled by elevating temperatures up to 75°C
- Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
- Rapid protocol saves two to three hours over conventional indirect methods
Order Info
Each system include the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500cm2 or 5000cm2 membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer and blocking agent
Specifications
| 2°C to 8°C | |
| Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes | |
| Labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer and blocking agent | |
| For 5000 cm2 membrane |