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Cytiva Amersham™ ECF™ Substrate for Western Blotting
Used for detection of protein blots with fluorescence scanning equipment. Cytiva Life Sciences™ Amersham™ ECF™ Substrate for Western Blotting uses a fluorescent substrate for alkaline phosphatase-based detection of protein blots.
Brand: Cytiva RPN5785
Includes: ECF Substrate, 60mL; Dilution buffer
Description
Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560nm when excited at the appropriate wavelength.
- For use with fluorescence scanning equipment
- Sensitivity comparable to chemiluminescence
- Wide dynamic range of fluorescence scanning systems compared with film allows visualization and quantitation of weak and dark bands in the same scan
- Eliminate need for darkroom and film
Western blotting, dot, and slot blotting
Specifications
| Fluorescence scanning equipment | |
| ECF Substrate, 60mL, Dilution Buffer | |
| Sufficient reagents for detecting 2500cm2 membrane |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Danger||
Hazard Category
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 1
- Specific target organ toxicity after repeated exposure Category 2
Hazard Statement
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H373-May cause damage to organs.
- EUH210-Safety data sheet available on request.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P313-Get medical advice/attention.
- P305-IF IN EYES:Immediately call a POISON CENTER/doctor..
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : 2,2'-iminodiethano
- UNKNOWN ACUTE-99.8 per cent of the mixture consists of component(s) of unknown acute toxicity.
- UNKNOWN AQUATIC-Contains 99.8% of components with unknown hazards to the aquatic environment.