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Cytiva Amersham™ ECL™ Direct Nucleic Acid Labeling/Detection System
Use for fast and easy detection of medium-to-high-target amounts. Cytiva Life Sciences™ Amersham™ ECL™ Direct Nucleic Acid Labeling/Detection System are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction
Brand: Cytiva RPN3005
Includes: 45-000-922 and 45-000-923: Labeling reagent; Crosslinker; Control DNA; Blocking agent; ECL detection reagents and ECL Gold hybridization buffer
Description
- Direct probe labeling in a 10 min reaction, 1 h from hybridization to detection with ECL Direct, Hybond N+, and Hyperfilm ECL.
- Eliminates handling, waste, and regulatory issues associated with the use of radioactivity.
- No need to strip blots before reprobing.
- For fast and easy detection of medium- to high-target amounts in applications such as colony/plaque screens, dot blots, and PCR product analyses.
- Consistent results combining strong signals with very low backgrounds.
Specifications
| 2°C to 8°C | |
| DNA labelling reagent 0.5mL; Glutaraldehyde solution 0.5mL; Water 1.0mL; l Hind III control DNA (10μg/mL) 0.1 ml; Gold hybridization buffer 500mL; Blocking agent 25g | |
| To LabeL 5 μg |
| Colony or plaque screens, dot blots and PCR product analyses | |
| ECL Direct Labeling Module | |
| 20 min. |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
- Danger
Hazard Category
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Skin sensitiser Category 1
- Respiratory sensitiser Category 1
- Specific target organ toxicity Category 3
- LONG-TERM AQUATIC HAZARD Chronic 3
Hazard Statement
- H319-Causes serious eye irritation.
- H315-Causes skin irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H317-May cause an allergic skin reaction.
- H335-May cause respiratory irritation.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P273-Avoid release to the environment.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- P405-Store locked up.
- P501-Dispose of contents/container to .