Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.
Brand: Thermo Scientific™ R0091
Thermo Scientific Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as 10 mM and 50 mM aqueous solutions titrated to pH 7.0 with NaOH.
Aminoallyl-dUTP can be enzymatically incorporated into DNA with:
• Reverse transcriptases
• Taq DNA polymerase (Cat. No. EP0072)
• phi29 DNA Polymerase (EP0091)
• Klenow Fragment (EP0051)
• Klenow Fragment, exo- (EP0421)
• DNA Polymerase I (EP0041)
The resulting amine-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin, or hapten.
Applications
• Enzymatic indirect non-radioactive labeling of DNA during cDNA synthesis
• PCR
• Nick-translation
• Random-primed labeling
• Primer extension
| Aminoallyl-dUTP Solution, 10 mM (100 μL) Store at –20°C. |
|
| DNA Polymerase | |
| Aminoallyl | |
| Dry Ice | |
| 10 mM |
| Liquid | |
| Direct Labeling | |
| 100 μL | |
| cDNA Synthesis, Non-Radioactive Labeling |
Catalog No. 10319879
Catalog No. 10520651
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For Research Use Only. Not for use in diagnostic procedures.
