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CD8 Mouse anti-Human, BD IMag-DM, Clone: SK1, BD
Mouse Monoclonal Antibody
Brand: BD Biosciences 557766
This item is not returnable.
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Description
Peripheral Blood Mononuclear Cells (PBMC) are labeled with BD IMag™ Anti-Human CD8 Magnetic Particles - DM according to the Magnetic Labeling Protocol. This labeled cell suspension is then placed within the magnetic field of the BD IMagnet™ (Cat. No. 552311). Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (negative fraction). The tube is then removed from the magnetic field for resuspension of the positive fraction. The separation is repeated twice to increase the purity of the positive fraction. The magnetic separation steps are diagrammed in the Separation Flow Chart. After the positive fraction is washed, the small size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications such as flow cytometry.
Specifications
| CD8 | |
| Monoclonal | |
| BD IMag-DM | |
| Aqueous buffered solution containing BSA and ≤0.09% sodium azide. | |
| Human Peripheral Blood T Cells | |
| 1 x 109 Cells | |
| Immunology | |
| Human | |
| IgG1 κ |
| Flow Cytometry | |
| SK1 | |
| CD8α; CD8A; CD8 alpha; Leu2a; MAL; T8; p32 | |
| Mouse | |
| Affinity Purified | |
| RUO | |
| Primary | |
| Store undiluted at 4°C |
For Research Use Only.