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BD Biosciences™ APO-BRDU™ Kit (RUO)
Two-color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry.
Brand: BD Biosciences™ 556405
This item is not returnable.
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Description
- Two-color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry.
- Sufficient reagents are provided to process 60 cell suspensions.
- The kit includes 5 mL of positive and 5 mL of negative control cell suspensions, approximately 1 x 10<sup>6</sup> cells per mL in 70% (v/v) ethanol.
- The control cells are derived from a human lymphoma cell line and have been fixed as described in the Fixation Protocol.
Specifications
| 60 Tests | |
| One kit/two parts: Part A - 0.30 mL bottle of FITC-Labeled Anti-BrdU mAb, 30 mL bottle of PI/RNase Staining Buffer, 0.6 mL vial of Reaction Buffer, 126 mL bottle of Rinsing Buffer, 120 mL bottle of Wash Buffer. Part B - 0.48 mL bottle of Br-dUTP, 5 mL bottle of Negative Control Cells, 5 mL bottle of Positive Control Cells, 0.045 mL bottle of TdT Enzyme. | |
| Part A (Comp. No. 51-6576AK) is shipped at ambient temperature and should be stored at 4°C upon arrival. Part B (Comp. No. 51-6576BK) is shipped at –80°C on dry ice, and should be stored at –20°C upon arrival. |
| One kit/two parts: Part A - 0.30 mL bottle of FITC-Labeled Anti-BrdU mAb, 30 mL bottle of PI/RNase Staining Buffer, 0.6 mL vial of Reaction Buffer, 126 mL bottle of Rinsing Buffer, 120 mL bottle of Wash Buffer. Part B - 0.48 mL bottle of Br-dUTP, 5 mL bottle of Negative Control Cells, 5 mL bottle of Positive Control Cells, 0.045 mL bottle of TdT Enzyme. | |
| 0.05% sodium azide | |
| Labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- APO-BRDU Kit
Signal Word
- Danger
Hazard Category
- Carcinogenicity Category 2
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 2
Hazard Statement
- H225-Highly flammable liquid and vapour.
- H319-Causes serious eye irritation.
- H351-Suspected of causing cancer.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol, cacodylic acid