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Applied Biosystems™ SYBR™ Select Master Mix
Cost-effective solution for real-time PCR applications.
Brand: Applied Biosystems™ 4472920
Includes: 2X mix contains SYBR GreenER dye, AmpliTaq DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components.
Description
- Specific - minimize primer-dimer and non-specific amplification
- Reproducible and sensitive - consistent amplification across a wide dynamic range
- Bright - contains SYBR™ GreenER™ dye for maximum brightness
- Carry-over contamination control - contains heat-labile UDG
- Can be used in either standard or fast cycling mode
Formulated for Maximum Specificity:
- SYBR™ Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix
- The master mix includes AmpliTaq™ DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity
Obtain Reproducible Results Across a Wide Dynamic Range:
- SYBR™ Select Master Mix is specially formulated to provide robust results from 100ng to 0.1pg cDNA per reaction and can reliably detect a single copy of genomic DNA
Contains SYBR™ GreenER™ Dye and Heat-labile UDG:
- SYBR™ GreenER™ dye is less inhibitory to PCR than SYBR™ Green I dye resulting in brighter signals
- Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control
Instrument Compatibility:
- SYBR™ Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments except the 7900HT real-time PCR system
- It is also compatible with the Bio-Rad IQ™5, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems
Order Info
Shipping Conditions: Wet Ice
Specifications
| The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 μL reactions (10 μL Master Mix per reaction). Store at 2–8°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems. |
|
| Liquid | |
| High | |
| AmpliTaq DNA Polymerase | |
| 7500 Fast System, 7500 System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Fast Mode, StepOne™, Standard Mode, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System, AB StepOnePlus™, Fast Mode, AB StepOne™, Standard Mode, AB 7500, Fast Mode, AB 7500, Standard Mode, AB StepOne™, Fast Mode, AB StepOnePlus™, Standard Mode | |
| ROX (Pre-mixed) | |
| Real Time PCR SYBR Master Mix | |
| 10 x 5 mL | |
| Gene Expression | |
| Built-In Hot Start | |
| DNA (Genomic), cDNA |
| SYBR | |
| Vial | |
| qPCR | |
| Fast or Standard | |
| SYBR GreenER | |
| SYBR™ | |
| UP (Ultra Pure) | |
| 100 Reactions of 20 μL | |
| 2X | |
| 5000 Reactions | |
| 10 x 5 mL |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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