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TaqMan™ TaqMan™ Gene Expression Master Mix
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Requires
Template and primers
TaqMan™ Gene Expression Master Mix has been validated with TaqMan™ Gene Expression Assays and Applied Biosystems™ real-time systems, providing precise quantification for a variety of gene expression-based real-time qPCR applications, including rare transcript detection, gene expression analysis, pathogen detection and viral load quantification,co-amplification of two targets, as well as specific detection of gene family members
TaqMan™ Gene Expression Master Mix contains AmpliTaq Gold™ DNA Polymerase, UP (Ultra Pure) for hot start activation and improved detection of bacterial targets, a blend of dNTPs with dTTP/dUTP and Uracil-DNA Glycosylase (UDG) to minimize carry-over PCR contamination, and a passive internal reference based on proprietary ROX™ dye for superb precision on Applied Biosystems™ real-time PCR instruments.
- Provides precise and reliable real-time qPCR quantification over nine logs of linear dynamic range
- TaqMan Gene Expression Master Mix is an optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target
- Extended benchtop stability provides consistent results for automated liquid-handling systems
- This makes it the choice for high-throughput sample processing overnight or over the weekend
- Validated with TaqMan gene expression assays and Applied Biosystems real-time systems, providing precise quantification for a variety of gene expression–based real-time qPCR applications
- Applications include rare transcript detection, gene expression analysis, pathogen detection and viral load quantification, co-amplification of two targets, as well as specific detection of gene family members
- Store at 2°C to 8°C
TaqMan Gene Expression Master Mix contains:
- AmpliTaq Gold DNA Polymerase, UP (Ultra Pure), for hot start activation and improved detection of bacterial targets
- A blend of dNTPs with dTTP/dUTP and uracil-DNA glycosylase (UDG) to minimize carry-over PCR contamination
- A passive internal reference based on proprietary ROX dye for superb precision on Applied Biosystems real-time PCR instruments
Informations sur la commande
Shipping Condition: Wet ice
Spécification
Spécification
| Content And Storage | 2 bottles (5 mL each) 2X TaqMan™ Gene Expression Master Mix sufficient for 400 reactions. Store at 2°C to 8°C. TaqMan™ Gene Expression Master Mix contains AmpliTaq Gold™ DNA Polymerase (Ultra Pure), Uracil-DNA glycosylase, dNTPs (with dUTP), ROX™ Passive Reference, and optimized buffer components. |
| Detection Method | Primer-probe |
| PCR Method | qPCR |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Reaction Speed | Standard |
| For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR SystemStepOne™, Standard Mode, StepOnePlus™, Standard Mode |
| Passive Reference Dye | ROX (Pre-mixed) |
| Product Line | TaqMan |
| Purity or Quality Grade | UP (Ultra Pure) |
| Quantity | 2 x 5 mL |
| Afficher plus |
For Research Use Only. Not for use in diagnostic procedures.
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