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BD Human IL-6 ELISA Kit II
Quantitative determination of human IL-6 in serum, plasma, and cell culture supernatant
Brand: BD 550799
Alert:
Toxic, avoid contact with skin, eyes, or clothing
Includes: 2 plates of 96 breakable wells coated with anti-human IL-6 antibody; Detection antibody: Biotinylated anti-human IL-6; Enzyme Concentrate (250X): Horseradish peroxidase conjugate; Recombinant human IL-6, lyophilized, 4 vials; Standard Diluent; Wash Concentrate (20X); Substrate Reagent A; Substrate Reagent B; Stop Solution; Adhesive Plate Sealers
Requires : Microplate reader to read at 450nm, precision pipettes (50 and 100μL), adjustable 1mL, 5mL, 10mL, 25mL pipettes, deionized or distilled water, wash bottle or automated microplate washer, log-log graph paper or computer and software for ELISA data analysis, tubes to prepare standard dilutions, laboratory timer, absorbent paper
This item is not returnable. View return policy
Description
IL-6 specific monoclonal antibody binds to immobilized antibody coated on 96-wells plate. Wells are washed and streptavidinhorseradish peroxidase conjugate mixed with biotinylated anti-human IL-6 antibody is added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IL-6 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450nm.
- Solid phase sandwich ELISA
- Standard Range: 4.7 - 300pg/mL
- Sensitivity: 4pg/mL
Specifications
| IL-6 | |
| 2 Plates of 96 breakable well coated with Anti-Human IL-6 Antibody, Biotinylated Anti-Human IL-6, HRP Conjugate (250X), 4 vials recombinant Human IL-6 (lyophilized), standard Diluent, wash concentrate (20X), Substrate reagent A, Substrate reagent B, stop | |
| Serum, Plasma, Cell Culture | |
| Quantitative determination of Human IL-6, ELISA |
| Human | |
| 2 Plates | |
| 2°C to 8°C | |
| Human IL-6 ELISA kit II |
Research use only