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Proteintech CoraLite™488 TUNEL Assay Apoptosis Detection Kit (green)
A distinctive feature of apoptosis is degradation of DNA.
Brand: Proteintech PF00006-20T
This item is not returnable.
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Description
- The DNA fragments tend to be vary in length in integer multiples of 180 bp-200 bp, which is shown as a ladder-like pattern in agarose gel electrophoresis.
- This kit uses the TUNEL method, using Terminal Deoxynucleotidyl Transferase (TdT) to catalyze the incorporation of CL488-dUTP at the 3'-OH end of the broken DNA of apoptotic cells.
- CL488-dUTP-labeled DNA can be directly observed with a fluorescence microscope or quantified with a flow cytometer.
- The TUNEL method can selectively detect apoptotic cells, but not necrotic cells or cells with DNA strand breaks caused by irradiation and drug treatment.
Specifications
| Emission: 515 nm | |
| 20 Tests | |
| Flow Cytometry | |
| Store at -20°C. Avoid exposure to light. Stable for 2 years after shipment. Avoid freeze- thaw cycles. |
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