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Cytiva CyDye™ DIFluors: Saturation Dye Labeling Kits
Allow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel
Brand: Cytiva 25-8009-84
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Description
- Size- and charge-matched dyes enable co-migration of labeled samples within the gel
- Bright and highly sensitive dyes allow the use of the minimal labeling technique
- Minimal loss of signal during labeling, separation, and scanning
- No change in signal over wide pH range used during first-dimension (IEF) separation
- Discrete signal from each fluor with minimal cross-talk contributes to high accuracy
Contain 100nm each Cy3 and Cy5 saturation dyes sufficient for at least 12 labeling reactions, using as little as 5μg protein per reaction. 25-8009-84 contains an additional vial of Cy3 saturation dye (400nm) for labeling up to 500μg of protein in a preparative gel.
For applications where only small amounts of sample are available, for example, in Laser Capture Microdissection.
Specifications
| Solid | |
| 100 nM each of Cy3, Cy5 and Extra Cy3 (400 nm) for preparative gels | |
| 100nM CyDye DIGE Fluor Cy3 saturation dye for analytical labeling, 100nM CyDye DIGE Fluor Cy5 saturation dye for analytical labeling, 400nM CyDye DIGE Fluor Cy3 saturation dye for preparative labeling | |
| Green, Red |
| For general 2D applications where sufficient amounts of sample are available | |
| Amersham CyDye DIGE Fluor Labeling Kit | |
| -15°C to -30°C |
Safety and Handling
Signal Word
Hazard Category
- Danger
Hazard Category
- Acute toxicity Category 3
- Skin sensitiser Category 1
- Respiratory sensitiser Category 1