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Invitrogen™ Dehydroepiandrosterone Sulfate (DHEA-S) Competitive ELISA Kit
Competitive ELISA
Brand: Invitrogen™ EIADHEAX10
Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, assay buffer, wash buffer, chromogen, stop solution, plate covers and lot-specific technical data sheet.
Description
The Dehydroepiandrosterone Sulfate (DHEA-S) ELISA quantitates DHEA-Se in serum,plasma,urine,saliva,dried fecal extracts or cell culture medium.
Principle of the method
The Dehydroepiandrosterone sulfate (DHEA-S) Competitive ELISA research-use-only kit uses a specifically generated antibody to measure Dehydroepiandrosterone sulfate (DHEA-S) in serum,plasma,urine,and saliva samples,and in fecal extracts. The kit will also quantitatively measure DHEA-S present in tissue culture media samples. A DHEA-S standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A DHEA-S-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to DHEA-S to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound DHEA-S-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.
Dehydroepiandrosterone sulfate (DHEA-S) is the major C19 steroid secreted by the adrenal cortex and a precursor in testosterone and estrogen biosynthesis. DHEA-S is identical across all species and so this kit should measure DHEA-S from all sources. It is produced by the addition of a sulfate group to dehydroepiandrosterone (DHEA), catalyzed by the sulfotransferase enzymes, SULT1A1 and SULT1E1, which also produce estrone sulfate from estrone. DHEA sulfate can also be back-converted to DHEA through the action of steroid sulfatase.
Specifications
| 96 to 60,000 pg/mL | |
| Dehydroepiandrosterone sulfate (DHEA-S) Competitive ELISA Kit | |
| HRP | |
| ELISA Kit | |
| 10 x 96 Assays | |
| ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti | |
| 8.4% | |
| Pre-coated 96-well plate, Standard, Assay Diluent Concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| RUO | |
| Fecal Extract (50 μL), Plasma (1 μL), Saliva (25 μL), Serum (1 μL), Supernatant (50 μL), Urine (25 μL) | |
| Chemical | |
| 2 hr. 30 min. |
| 90.9 pg/mL | |
| Multiplex Assay on the Luminex Platform | |
| Fluorescence | |
| Fecal Extract, Plasma, Saliva, Serum, Supernatant, Urine | |
| Human | |
| Colorimetric Microplate Reader | |
| 7% | |
| HRP | |
| Reproductive Biology | |
| -20°C | |
| 1 hr. 20 min. |