Macherey-Nagel™ Dummy Strip

Description

The NucleoSpin™ Multi-8 Plasmid kit is designed for purifying up to 25μg of high copy-number plasmid DNA from 1mL to 5mL E. coli over night cultures preferably grown in LB medium.
The NucleoSpin™ Plasmid binding strips contain a unique silica membrane that binds DNA in the presence of chaotropic salt. After a modified alkaline lysis procedure, bacterial lysates are clarified by centrifugation. Cleared lysates are loaded onto individual wells of the plasmid binding strips arranged on the NucleoVac 96 vacuum manifold. The washing steps are followed by drying the membrane and low-salt elution (5mM Tris/HCl, pH 8.5) of highly pure plasmid DNA which is ready to use and suitable for eg. radioactive and fluorescent sequencing, library screening and cloning. Alternatively, water (pH 8.5) can be used for elution.Kit components: NucleoSpin™ Plasmid binding strips, buffers, RNase A, MN wash plate, MN tube strips, cap strips.For further information on this product please contact Customer Services.
Silica membrane technology

• Yield: up to 25μg sequencing grade plasmid DNA
• Elution volume: 75μL to 150μL
• Culture volume: 1mL to 5mL
• Binding capacity: 25μg
• Maximum plasmid size <15kb
• No use of organic solvents

Rapid small-scale preparation of high copy plasmids from E.coli.