missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Invitrogen™ Dynabeads™ SARS-CoV-2 Spike Ig Total ELISA Kit
The Dynabeads™ SARS-CoV-2 Spike Ig Total ELISA Kit is a solid‐phase sandwich enzyme‐linked immunosorbent assay (ELISA) designed to detect and quantify the level of human SARS-CoV-2 IgG, IgM, and IgA in serum and plasma samples. The kit can be used for both qualitative and quantitative assays.
Brand: Invitrogen™ 18020D
This item is not returnable.
View return policy
Description
- A solid‐phase sandwich enzyme‐linked immunosorbent assay (ELISA) designed to detect and quantify the level of human SARS-CoV-2 IgG, IgM, and IgA in serum and plasma samples
- The steps required for use are simply to pre-dilute the reagents, fill the wells of the KingFisher plastic plates, and push start on the instrument
- After the run, the sample is transferred to a plate compatible with the absorbance reader and the OD is read at 450 nm
- A DynaMag-96 well magnet can be used while still taking advantage of the faster binding kinetics of magnetic beads compared to the more traditional plate-bound ELISAs
- Kit contains all buffers required, as well as calibrator antibodies, HRP-conjugated detection antibodies, substrate solution, beads, and stop solution sufficient for one 96-well plate
- Dynabeads SARS-CoV-2 Spike beads can be purchased separately (18100D) for other uses such as the optimization of unique conditions
- Using Dynabeads and automation drastically improves reproducibility, limits hands on time, and reduces operating errors
- The intra-assay CV value is typically <5% (range 2.8–3.2%), while the inter-assay CV is <15% (range 3.9–9.3%)
- Kit can be used for both qualitative and quantitative assays
- One of the fastest ELISAs for SARS-CoV-2 serology
- For research use only, not for use in diagnostic procedures
Specifications
| ISO 9001 | |
| Absorbance at 450 nm | |
| Binding of human immunoglobulins (IgG, IgM, IgA) towards SARS-CoV-2 (Spike trimer) | |
| 96 reactions | |
| 2.7 μm | |
| Yes, on KingFisher™ Flex or Apex instruments |
| • Dynabeads SARS-CoV-2 Spike, 3 mL (1 mg/mL) • Human SARS-CoV-2 total Ig High Control antibody, 1 vial (lyophilized) • Human SARS-CoV-2 Total Ig Low Control antibody, 1 vial (lyophilized) • Human SARS-CoV-2 Total Ig Calibrator (medium control) antibody, 1 vial (lyophilized) • Wash buffer (20X concentrated), 50 mL • Assay buffer (20X concentrated), 50 mL • Substrate solution (tetramethylbenzidine), 15 mL • Stop solution, 15 mL • Human SARS-CoV-2 Total Ig HRP Conjugate, 0.12 mL Store at 2–8°C. |
|
| SARS-CoV-2 ELISA Ig Total Kit | |
| KingFisher™ Flex or Apex Instruments, DynaMag™-96 Side Skirted, Magnetic 96-Well Separator, ELISA Plate Reader (450 nm) | |
| Wet ice (2° to 8°C) | |
| Monosized, superparamagnetic polystyrene magnetic beads |
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Invitrogen™ Dynabeads™ SARS-CoV-2 Spike Ig Total ELISA Kit > 96
For research use only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?Share a Content Correction