Invitrogen™ Dynabeads™ Streptavidin for In Vitro Transcription

Description

Dynabeads Streptavidin for In Vitro Transcription offers an innovative technology for simple, flexible, and automation-ready mRNA synthesis in scales ranging from microgram to gram. The 1-μm monosized, monodispersed, paramagnetic beads are covalently coupled to streptavidin molecules, ready for binding to a biotinylated DNA template. The workflow is designed to minimize the effort and cost of plasmid preparation while enabling storage and reuse of bead-template complex in a successive solid-phase in vitro transcription (IVT) reaction.

Benefits of Dynabeads Streptavidin for In Vitro Transcription beads include:

• Flexibility—choose a DNA template for IVT mRNA with greater flexibility of design and amplification
• Efficiency—reuse the DNA template (>6x) to reduce cost and minimize plasmid prep hands-on time, and reduce the risk of transfer of bacterial host DNA
• Scalability—swap from manual to automated workflow to match the required scale and throughput
• Quality—helps ensure a safe and flexible supply of magnetic beads to navigate from RNA-based drug discovery to commercial manufacturing without incurring extra time or costs from switching technology or raw materials

Reusable bead-based IVT

The biotinylated template is made by PCR amplification of the target sequence in a plasmid or a synthetic DNA construct. This uses a biotinylated forward primer, located 30-100 base pairs upstream of the T7-promoter, and a non-biotinylated reverse primer. As an alternative to PCR amplification, a linearized plasmid can be biotinylated by a fill-in reaction of biotin-dUTP in the 5' overhang sequence. The biotinylated template is then immobilized directly to Dynabeads Streptavidin for IVT without the need for prior purification. The bead-bound template is used directly in the IVT, then removed from the synthesized mRNA by magnetic separation. The template can then be reused at least six times in consecutive IVT reactions. The protocol is scalable from micrograms to grams of mRNA.

Automation ready with the KingFisher Purification System

The 1-μm Dynabeads magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of the KingFisher Sample Purification Systems or liquid handlers.

Commercial supply

Our manufacturing sites are ISO 13485-certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply. If you are in the process of customizing Dynabeads Streptavidin for In Vitro Transcription beads in a commercial product or service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or read more on our Dynabeads OEM page.

To learn about other solutions and support documentation available for GMP-compliant manufacturing of mRNA vaccines and therapeutics visit thermofisher.com/dynabeadsmrna.