Invitrogen™ E-Gel™ Double Comb Agarose Gels with SYBR™ Safe DNA Gel Stain, 1%

Description

Invitrogen™ E-Gel™ Double Comb Agarose Gels with SYBR Safe DNA Gel Stain are designed for fast, convenient, and less-hazardous DNA sample electrophoresis. Each E-Gel™ Double Comb gel cassette contains all components and stain required for efficient gel separation and analysis—just load your samples and run. E-Gel™ Double Comb gels are ideal for analyzing PCR products, restriction digests, plasmid preparations, or genotyping samples.

Features of E-Gel™ Double Comb Agarose Gels include:

• Instantaneous setup—ready for use when you are and no gel preparation or additional buffers required
• Less-hazardous choice—contains SYBR Safe DNA stain, a less-hazardous alternative to ethidium bromide
• High performance—detect 5 ng of sample DNA with a separation time as short as 13-18 minutes
• In-process control—view DNA sample migration in real time
• Easy sample access—openable cassette design allows easy in-gel access for convenient DNA sample excision or transfer to membranes
• Better sample integrity—exploits blue-light excitation wavelength to minimize DNA damage, as opposed to UV

Less hazardous option

Choose SYBR Safe DNA gel stain to minimize exposure to hazardous and mutagenic options such as ethidium bromide or UV light. Precast E-Gel™ Double Comb gels with SYBR Safe stain, in combination with blue-light trans-illumination, offer better sample integrity and a less hazardous work environment.

Fast and convenient analysis

E-Gel™ agarose gels enable fast and high-performance analysis with a detection sensitivity of 5 ng of sample DNA per band and a separation time 2–3 fold faster than that of conventional pour-your-own gels. Each E-Gel™ Double Comb gel contains agarose, electrodes, SYBR Safe DNA stain, and a buffer-less ion exchange system packaged inside a dry disposable cassette. E-Gel™ technology does not require any gel or buffer preparation or gel staining steps. Just load your samples and run.

Live monitoring of DNA migration

E-Gel™ Double Comb gels include SYBR Safe DNA Gel Stain, which has an excitation wavelength in the blue-light spectrum. When used on the E-Gel™ Power Snap Electrophoresis Device with integrated blue-light trans-illuminator, the gels enable less hazardous real-time monitoring of DNA migration without risk to the eyes or risk of damaging the sample, as opposed to UV visualization.

Easy access to the gel

E-Gel™ Double Comb cassettes are designed for convenient opening with a Gel Knife (Cat. No. EI9010), so that the gel inside can be accessed readily for excision of specific bands or transferred to a membrane for southern blot analysis.

Nucleic acid markers

For optimal DNA analysis using E-Gel™ Double Comb gels with SYBR Safe DNA stain, we recommend the specially formulated E-Gel™ 1 Kb Plus Express DNA Ladder (Cat. No: 10488091), E-Gel™ 96 High Range DNA Marker (Cat. No. 12352019), or E-Gel™ Low Range Quanitative DNA Ladder (Cat. No. 12373031). Our high-quality ready-to-use ladders are formulated using individual chromatography-purified DNA fragments and are suitable for storage at room temperature.

Running and imaging devices

E-Gel™ Double Comb gels require the E-Gel™ Power Snap Electrophoresis System for gel running and viewing. The system consists of an electrophoresis device and a camera for fast and convenient E-Gel™ agarose gel separation and analysis.