Invitrogen™ Estrone-3-Glucuronide (E1G) Competitive ELISA Kit

Description

The Estrone-3 Glucuronide (E1G) ELISA quantitates estrone-3 glucuronide in extracted serum,plasma,urine,dried fecal extracts or cell culture medium.

Principle of the method

The Estrone-3-Sulfate (E1S) Competitive ELISA research-use-only kit uses a specifically generated antibody to measure Estrone-3-Sulfate (E1S) in a variety of matrices,including serum,plasma,urine,and fecal samples. The kit will quantitatively measure E1S present in diluted buffer samples and tissue culture media samples. An E1S standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An E1S-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to E1S to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound E1S-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.