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Thermo Scientific™ Exonuclease I (20 U/μL)

Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.

Brand: Thermo Scientific™ EN0582

295.32 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.

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Product Code. 10354530

428.00€ / 20000 units

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Description

Exonuclease I (ExoI) degrades single-stranded DNA in a 3'≥5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.

It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.

Highlights

Active in PCR buffers

Applications

Primer removal from PCR mixtures:
- prior to PCR product sequencing
Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus

Note

The enzyme is not suitable for removing 3'-overhangs of dsDNA.

Specifications

Concentration	20 U/μL
Enzyme	Exonuclease
Quantity	20,000 U
Product Type	Exonuclease I

Product Suggestions Safety and Handling

Safety and Handling

Product Identifier

10X Reaction Buffer for Exonuclease I

Signal Word

Warning

Hazard Category

Serious eye damage/eye irritation Category 2
Skin corrosion/irritation Category 2

Hazard Statement

H315-Causes skin irritation.
H319-Causes serious eye irritation.

Precautionary Statement

P264-Wash hands thoroughly after handling.
P280-Wear protective gloves/protective clothing/eye protection/face protection.
P302+P352-IF ON SKIN: Wash with plenty of water/soap.
P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P332+P313-If skin irritation occurs: Get medical advice/attention.
P337+P313-If eye irritation persists: Get medical advice/attention.
P362+P364-Take off contaminated clothing and wash it before reuse.

Supplemental information

MIXTURE LIST-Contains : Potassium hydroxide

For Research Use Only. Not for use in diagnostic procedures.